| Autor: |
Nabel AL; Division of Neurobiology, Department Biology II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.; Graduate School for Systemic Neurosciences, Ludwig-Maximilians-Universität München, Munich, Germany., Callan AR; Division of Neurobiology, Department Biology II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.; Graduate School for Systemic Neurosciences, Ludwig-Maximilians-Universität München, Munich, Germany., Gleiss SA; Division of Neurobiology, Department Biology II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.; Graduate School for Systemic Neurosciences, Ludwig-Maximilians-Universität München, Munich, Germany., Kladisios N; Institute of Zoology, University of Veterinary Medicine Hannover, Hanover, Germany., Leibold C; Computational Neuroscience, Department Biology II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany., Felmy F; Division of Neurobiology, Department Biology II, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.; Institute of Zoology, University of Veterinary Medicine Hannover, Hanover, Germany. |
| Abstrakt: |
Coincidence detector neurons of the medial superior olive (MSO) are sensitive to interaural time differences in the range of a few tens of microseconds. The biophysical basis for this remarkable acuity is a short integration time constant of the membrane, which is achieved by large low voltage-activated potassium and hyperpolarization-activated inward cation conductances. Additional temporal precision is thought to be achieved through a sub-cellular distribution of low voltage-activated potassium channel expression biased to the soma. To evaluate the contribution of potassium channels, we investigated the presence and sub-cellular distribution profile of seven potassium channel sub-units in adult MSO neurons of gerbils. We find that low- and high voltage-activated potassium channels are present with distinct sub-cellular distributions. Overall, low voltage-activated potassium channels appear to be biased to the soma while high voltage-activated potassium channels are more evenly distributed and show a clear expression at distal dendrites. Additionally, low voltage-activated potassium channel sub-units co-localize with glycinergic inputs while HCN1 channels co-localize more with high voltage-activated potassium channels. Functionally, high voltage-activated potassium currents are already active at low voltages near the resting potential. We describe a possible role of high voltage-activated potassium channels in modulating EPSPs in a computational model and contributing to setting the integration time window of coincidental inputs. Our data shows that MSO neurons express a large set of different potassium channels with distinct functional relevance. |
