Synergistic activity of colistin with azidothymidine against colistin-resistant Klebsiella pneumoniae clinical isolates collected from inpatients in Greek hospitals.

Autor: Falagas ME; Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece; Department of Medicine, Henry Dunant Hospital Center, Athens, Greece; Department of Medicine, Tufts University School of Medicine, Boston, MA, USA. Electronic address: m.falagas@aibs.gr., Voulgaris GL; Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece; Laboratory of Pharmacokinetics and Toxicology, Department of Pharmacy, 401 General Military Hospital, Athens, Greece., Tryfinopoulou K; Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece., Giakkoupi P; Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece., Kyriakidou M; Alfa Institute of Biomedical Sciences (AIBS), Athens, Greece., Vatopoulos A; Department of Microbiology, National School of Public Health; Central Public Health Laboratory, Hellenic Centre of Disease Control and Prevention, Vari, Greece., Coates A; Institute for Infection and immunity, St. George's, University of London, London, UK., Hu Y; Institute for Infection and immunity, St. George's, University of London, London, UK.
Jazyk: angličtina
Zdroj: International journal of antimicrobial agents [Int J Antimicrob Agents] 2019 Jun; Vol. 53 (6), pp. 855-858. Date of Electronic Publication: 2019 Mar 02.
DOI: 10.1016/j.ijantimicag.2019.02.021
Abstrakt: Background: New antibiotics are urgently needed to treat multi-drug resistant infections; however, production of novel antibiotics is diminishing. Synergistic combination drug therapy to enhance the activity of available antibiotics may improve management of patients with resistant infections.
Methods: Colistin-resistant Klebsiella pneumoniae isolates were collected from inpatients in 10 Greek hospitals and used to study combination activity of colistin plus azidothymidine. Combination activity was evaluated with the sum of fractional inhibitory concentrations (ΣFIC) using the mini checkerboard broth microdilution method.
Results: A hundred individual strains were tested. Synergistic activity was noted in 79% (79/100) of isolates and additive activity in the remaining 21% (21/100). ΣFIC 50 and ΣFIC 90 were 0.28 and 0.56, respectively.
Conclusion: Colistin with azidothymidine exhibited promising synergistic activity against colistin-resistant Klebsiella pneumoniae isolates warranting further investigation of the combination.
(Copyright © 2019 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.)
Databáze: MEDLINE
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