1,25(OH) 2 D 3 deficiency-induced gut microbial dysbiosis degrades the colonic mucus barrier in Cyp27b1 knockout mouse model.

Autor: Zhu W; 1Department of Anatomy, Histology and Embryology, Nanjing Medical University, Xuehai Building, Rm D509, 101 Longmian Avenue, Jiangning District, Nanjing, 211166 China., Yan J; 2Department of Clinical Medicine, First Clinical Medicine College, Nanjing Medical University, Nanjing, 211166 China., Zhi C; 4Department of Pathology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002 China., Zhou Q; 1Department of Anatomy, Histology and Embryology, Nanjing Medical University, Xuehai Building, Rm D509, 101 Longmian Avenue, Jiangning District, Nanjing, 211166 China., Yuan X; 1Department of Anatomy, Histology and Embryology, Nanjing Medical University, Xuehai Building, Rm D509, 101 Longmian Avenue, Jiangning District, Nanjing, 211166 China.; 3Key Laboratory for Aging & Disease, Nanjing Medical University, Nanjing, 211166 China.
Jazyk: angličtina
Zdroj: Gut pathogens [Gut Pathog] 2019 Feb 20; Vol. 11, pp. 8. Date of Electronic Publication: 2019 Feb 20 (Print Publication: 2019).
DOI: 10.1186/s13099-019-0291-z
Abstrakt: Background: The relationship between disturbances of the gut microbiota and 1,25(OH) 2 D 3 deficiency has been established both in humans and animal models with a vitamin D poor diet or a lack of sun exposure. Our prior study has demonstrated that Cyp27b1 - / - ( Cyp27b1 knockout) mice that could not produce 1,25(OH) 2 D 3 had significant colon inflammation phenotypes. However, whether and how 1,25(OH) 2 D 3 deficiency due to the genetic deletion controls the gut homeostasis and modulates the composition of the gut microbiota remains to be explored.
Results: 1,25(OH) 2 D 3 deficiency impair the composition of the gut microbiota and metabolite in Cyp27b1 - / - mice, including Akkermansia muciniphila, Solitalea Canadensis , Bacteroides - acidifaciens , Bacteroides plebeius and SCFA production. 1,25(OH) 2 D 3 deficiency cause the thinner colonic mucus layer and increase the translocation of the bacteria to the mesenteric lymph nodes. We also found 1,25(OH) 2 D 3 supplement significantly decreased Akkermansia muciniphila abundance in fecal samples of Cyp27b1 - / - mice.
Conclusion: Deficiency in 1,25(OH) 2 D 3 impairs the composition of gut microbiota leading to disruption of intestinal epithelial barrier homeostasis and induction of colonic inflammation. This study highlights the association between 1,25(OH) 2 D 3 status, the gut microbiota and the colonic mucus barrier that is regarded as a primary defense against enteric pathogens.
Databáze: MEDLINE
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