Determination of Cell-Surface Hyaluronan Through Flow Cytometry.

Autor: Vitale DL; Laboratorio de Microambiente Tumoral, Universidad Nacional del Noroeste de la Pcia. de Bs. As, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA UNNOBA-CONICET), Junín, Buenos Aires, Argentina., Spinelli FM; Laboratorio de Microambiente Tumoral, Universidad Nacional del Noroeste de la Pcia. de Bs. As, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA UNNOBA-CONICET), Junín, Buenos Aires, Argentina., Alaniz L; Laboratorio de Microambiente Tumoral, Universidad Nacional del Noroeste de la Pcia. de Bs. As, Centro de Investigaciones y Transferencia del Noroeste de la Provincia de Buenos Aires (CITNOBA UNNOBA-CONICET), Junín, Buenos Aires, Argentina. ldalaniz@comunidad.unnoba.edu.ar.
Jazyk: angličtina
Zdroj: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1952, pp. 111-116.
DOI: 10.1007/978-1-4939-9133-4_10
Abstrakt: Hyaluronan is the major glycosaminoglycan present in the extracellular matrix of several cell types; its synthesis occurs on the cellular plasma membrane. Variations in its expression are related to alterations in cell proliferation, adhesion, and migration. It is able to interact with different binding proteins called hyaladherins, which can be conjugated to different fluorochromes and analyzed by flow cytometry.
Databáze: MEDLINE