| Autor: |
Chistyakov DV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Azbukina NV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Goriainov SV; Peoples` Friendship University of Russia, Moscow, Russia., Chistyakov VV; Peoples` Friendship University of Russia, Moscow, Russia., Gancharova OS; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Tiulina VV; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Baksheeva VE; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Iomdina EN; Moscow Helmholtz Research Institute of Eye Diseases, Moscow, Russia., Philippov PP; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Sergeeva MG; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Senin II; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia., Zernii EY; Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia. |
| Abstrakt: |
The ultraviolet (UV) B-induced damage of the eye surface of experimental animals (rabbits) includes loss of corneal epithelium, apoptosis of keratocytes and stromal edema. These changes are accompanied by clinically and histologically manifested corneal inflammation, neutrophil infiltration, and exudation of the anterior chamber of the eye. According to mass spectrometric analysis, UV-induced corneal damage is associated with pronounced changes in the lipid composition of tears, including a decrease in the amount of arachidonic acid and prostaglandin E2 and an increase in the concentrations of prostaglandin D2 and its derivative 15d-PGJ2. In addition, it is accompanied by an alteration in the levels of hydroxyeicosate tetraenic acid derivatives, namely upregulation of 12-HETE and downregulation of 5-HETE. The revealed changes indicate the activation of metabolic pathways involving 5-lipoxygenase, 12-lipoxygenase, cyclooxygenase 1 and 2, and prostaglandin-D-synthase. These findings contribute to understanding mechanisms of UV-induced keratitis and point on feasibility of selective anti-inflammatory therapy for improving corneal regeneration after iatrogenic UV damage. |
