Immobilization of Denosumab on Titanium Affects Osteoclastogenesis of Human Peripheral Blood Monocytes.

Autor: Beck F; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. Huber.felicitas@googlemail.com., Hartmann ES; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. elizahartmann@msn.com., Koehler MI; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. miriam.koehler@gwweb.de., Redeker JI; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. julia.redeker@icloud.com., Schluessel S; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. Sabine.Schluessel@med.uni-muenchen.de., Schmitt B; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. baerbel.schmitt@med.uni-muenchen.de., Fottner A; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. andreas.fottner@med.uni-muenchen.de., Unger M; Experimental Trauma Surgery, Department of Trauma Surgery, Klinikum Rechts der Isar, Technical University of Munich, Ismaninger Strasse 22, 81675 Munich, Germany. unger@uchir.me.tum.de., van Griensven M; Experimental Trauma Surgery, Department of Trauma Surgery, Klinikum Rechts der Isar, Technical University of Munich, Ismaninger Strasse 22, 81675 Munich, Germany. martijn.vangriensven@tum.de., Michael J; Institut für Korrosionsschutz, Dresden GmbH, Gostritzer Straße 65, 01217 Dresden, Germany. jannaj@gmx.de., Summer B; Department of Dermatology and Allergology, Ludwig-Maximilians-University, Frauenlobstr. 9-11, 80337 Munich, Germany. burkhard.summer@med.uni-muenchen.de., Kunzelmann KH; Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Goethestraße 70, 80336 Munich, Germany. Karl-Heinz.Kunzelmann@med.uni-muenchen.de., Beutner R; Institute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden, Budapester Straße 27, 01069 Dresden, Germany. rene.beutner@tu-dresden.de., Scharnweber D; Institute of Materials Science, Max Bergmann Center of Biomaterials, TU Dresden, Budapester Straße 27, 01069 Dresden, Germany. dieter.scharnweber@tu-dresden.de., Kostenuik PJ; Phylon Pharma Services, Newbury Park, CA 91320, USA. PKost@PhylonPS.com.; School of Dentistry, University of Michigan, Ann Arbor, MI 48109, USA. PKost@PhylonPS.com., Mayer-Wagner S; Department of Orthopaedics, Physical Medicine and Rehabilitation, University Hospital, LMU Munich, Marchioninistraße 15, 81377 Munich, Germany. Susanne.mayer@med.uni-muenchen.de.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2019 Feb 26; Vol. 20 (5). Date of Electronic Publication: 2019 Feb 26.
DOI: 10.3390/ijms20051002
Abstrakt: Immobilization of proteins has been examined to improve implant surfaces. In this study, titanium surfaces were modified with nanofunctionalized denosumab (cDMAB), a human monoclonal anti-RANKL IgG. Noncoding DNA oligonucleotides (ODN) served as linker molecules between titanium and DMAB. Binding and release experiments demonstrated a high binding capacity of cDMAB and continuous release. Human peripheral mononuclear blood cells (PBMCs) were cultured in the presence of RANKL/MCSF for 28 days and differentiated into osteoclasts. Adding soluble DMAB to the medium inhibited osteoclast differentiation. On nanofunctionalized titanium specimens, the osteoclast-specific TRAP5b protein was monitored and showed a significantly decreased amount on cDMAB-titanium in PBMCs + RANKL/MCSF. PBMCs on cDMAB-titanium also changed SEM cell morphology. In conclusion, the results indicate that cDMAB reduces osteoclast formation and has the potential to reduce osteoclastogenesis on titanium surfaces.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje