Effect of strontium substitution on the material properties and osteogenic potential of 3D powder printed magnesium phosphate scaffolds.

Autor: Meininger S; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany., Moseke C; Institute for Biomedical Engineering (IBMT), University of Applied Sciences Mittelhessen (THM), Gießen, Germany., Spatz K; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany., März E; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany., Blum C; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany., Ewald A; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany., Vorndran E; Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Germany. Electronic address: elke.vorndran@fmz.uni-wuerzburg.de.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 May; Vol. 98, pp. 1145-1158. Date of Electronic Publication: 2019 Jan 15.
DOI: 10.1016/j.msec.2019.01.053
Abstrakt: 3D powder printing is a versatile method for the fabrication of individual bone implants and was used for the processing of in vivo degradable ceramic scaffolds based on ammonium magnesium phosphate hexahydrate (struvite). In this study, synergetic effects could be achieved by the substitution of magnesium phosphate cements with strontium carbonate. This substitution resulted in 8.2 wt%, 16.4 wt%, and 24.6 wt% Sr 2+ doped scaffolds, with a 1.9-3.1 times increased radiopacity compared to pure struvite. The maximal compressive strength of (16.1 ± 1.1) MPa found for strontium substituted magnesium phosphate was in the range of cancelleous bone, which makes these 3D printed structures suitable for medical application in low-load-bearing bone areas. In an ion release study over a course of 18 days, the release of strontium, magnesium, calcium, and phosphate ions from scaffolds was analyzed by means of inductively coupled plasma mass spectrometry. Independent of the scaffold composition the Mg 2+ concentrations (83-499 mg/l) continuously increased in the cell media. The Sr 2+ release varied between 4.3 μg/day and 15.1 μg/day per g scaffold, corresponding to a Sr 2+ concentration in media between 1.14 mg/l and 7.24 mg/l. Moreover, decreasing calcium and phosphate concentrations indicated the precipitation of an amorphous calcium phosphate phase. The superior osteogenic properties of strontium substituted magnesium phosphate, e.g. the increase of osteoblast activity and cell number and the simultaneous suppression of osteoclast differentiation could be verified in vitro by means of WST-assay, TRAP-staining, and SEM imaging.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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