| Autor: |
Tohya M; 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Watanabe S; 2Department of Microbiome Research, Juntendo University School of Medicine, Tokyo, Japan., Teramoto K; 3Koichi Tanaka Mass Spectrometry Research Laboratory, Kyoto, Japan., Uechi K; 4Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan., Tada T; 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Kuwahara-Arai K; 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Kinjo T; 4Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan., Maeda S; 5Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan., Nakasone I; 4Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan., Zaw NN; 6Clinical Pathology Section, Mandalay General Hospital, Mandalay, Myanmar., Mya S; 7National Health Laboratory, Yangon, Myanmar., Zan KN; 7National Health Laboratory, Yangon, Myanmar., Tin HH; 7National Health Laboratory, Yangon, Myanmar., Fujita J; 4Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan., Kirikae T; 1Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan. |
| Abstrakt: |
A novel Gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, RYU5 T , was isolated from a stool sample of an inpatient at a hospital in Okinawa, Japan. The optimal growth temperature of RYU5 T was 30 °C. Phylogenetic analysis based on the sequences of housekeeping genes, including the 16S rRNA, rpoB, rpoD and gyrB genes, showed that RYU5 T was a member of the Pseudomonas putida group and was located close to Pseudomonas monteilii and P. putida. Whole-genome comparisons, using average nucleotide identity and digital DNA-DNA hybridization, confirmed that strain RYU5 T should be classified as a novel species of Pseudomonas. Phenotypic characterization tests showed that utilization of d-mannose, d-serine, l-arabinose and d-fructose could distinguish this strain from other related species of the genus Pseudomonas. Based on genetic and phenotypic evidence, strain RYU5 T should be classified as a novel species, for which the name Pseudomonas asiatica sp. nov. is proposed. The type strain is RYU5 T (=DSM 107182 T , =JCM 32716 T ), with a DNA G+C content of 62.25 mol%. |
