C-terminal regulatory domain of the ε subunit of F o F 1 ATP synthase enhances the ATP-dependent H + pumping that is involved in the maintenance of cellular membrane potential in Bacillus subtilis.

Autor: Akanuma G; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan.; Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan., Tagana T; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan., Sawada M; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan., Suzuki S; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan., Shimada T; Laboratory for Chemistry and Life Science, Institute of Innovative Science, Tokyo Institute of Technology, Yokohama, Midori-ku, Japan., Tanaka K; Laboratory for Chemistry and Life Science, Institute of Innovative Science, Tokyo Institute of Technology, Yokohama, Midori-ku, Japan., Kawamura F; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan., Kato-Yamada Y; Department of Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan.; Research Center for Life Science, College of Science, Rikkyo University, Toshima-ku, Tokyo, Japan.
Jazyk: angličtina
Zdroj: MicrobiologyOpen [Microbiologyopen] 2019 Aug; Vol. 8 (8), pp. e00815. Date of Electronic Publication: 2019 Feb 27.
DOI: 10.1002/mbo3.815
Abstrakt: The ε subunit of F o F 1 -ATPase/synthase (F o F 1 ) plays a crucial role in regulating F o F 1 activity. To understand the physiological significance of the ε subunit-mediated regulation of F o F 1 in Bacillus subtilis, we constructed and characterized a mutant harboring a deletion in the C-terminal regulatory domain of the ε subunit (ε ∆C ). Analyses using inverted membrane vesicles revealed that the ε ∆C mutation decreased ATPase activity and the ATP-dependent H + -pumping activity of F o F 1 . To enhance the effects of ε ∆C mutation, this mutation was introduced into a ∆rrn8 strain harboring only two of the 10 rrn (rRNA) operons (∆rrn8 ε ∆C mutant strain). Interestingly, growth of the ∆rrn8 ε ∆C mutant stalled at late-exponential phase. During the stalled growth phase, the membrane potential of the ∆rrn8 ε ∆C mutant cells was significantly reduced, which led to a decrease in the cellular level of 70S ribosomes. The growth stalling was suppressed by adding glucose into the culture medium. Our findings suggest that the C-terminal region of the ε subunit is important for alleviating the temporal reduction in the membrane potential, by enhancing the ATP-dependent H + -pumping activity of F o F 1 .
(© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
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