Liposome and polymeric micelle-based delivery systems for chlorophylls: Photodamage effects on Staphylococcus aureus.

Autor: Gerola AP; Chemistry Department, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil; Chemistry Department, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, 88040-900, Brazil. Electronic address: adriana.gerola@ufsc.br., Costa PFA; Chemistry Department, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil; Chemistry Department, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, 88040-900, Brazil., de Morais FAP; Chemistry Department, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Tsubone TM; Institute of Chemistry, Universidade de São Paulo, São Paulo, São Paulo, 05508-000, Brazil., Caleare AO; Department of Clinical Analyzes and Biomedicine, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Nakamura CV; Department of Physiological Sciences, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Brunaldi K; Department of Pharmacy and Pharmacology, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Caetano W; Chemistry Department, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Kimura E; Department of Pharmacy and Pharmacology, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil., Hioka N; Chemistry Department, Universidade Estadual de Maringá, Maringá, Paraná, 87020-900, Brazil.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2019 May 01; Vol. 177, pp. 487-495. Date of Electronic Publication: 2019 Feb 18.
DOI: 10.1016/j.colsurfb.2019.02.032
Abstrakt: Chlorophyll derivatives (Chls), loaded in F-127 polymeric micelles and DPPC liposomes as drug delivery systems (DDS), have been shown to be remarkable photosensitizers for photodynamic inactivation (PDI). Assays of photoinactivation of Staphylococcus aureus bacteria (as biological models) showed that the effectiveness of Chls in these nanocarriers is dependent on photobleaching processes, photosensitizer locations in DDS, singlet oxygen quantum yields, and Chl uptake to bacteria. These are factors related to changes in Chl structure, such as the presence of metals, charge, and the phytyl chain. The photodynamic activity was significantly greater for Chls without the phytyl chain, i.e., phorbides derivatives. Furthermore, the inactivation of S. aureus was increased by the use of liposomes compared to micelles. Therefore, this research details and shows the high significance of the Chl structure and delivery system to enhance the photodynamic activity. It also highlights the chlorophylls (particularly phorbides) in liposomes as promising photosensitizers for PDI.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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