Effects of a polysaccharide-rich extract derived from Irish-sourced Laminaria digitata on the composition and metabolic activity of the human gut microbiota using an in vitro colonic model.

Autor: Strain CR; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine, BT52 1SA, UK.; Teagasc Food Research Centre, Moorepark, Fermoy, Ireland.; APC Microbiome Institute, University College Cork, Cork, Ireland., Collins KC; Teagasc Food Research Centre, Moorepark, Fermoy, Ireland., Naughton V; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine, BT52 1SA, UK., McSorley EM; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine, BT52 1SA, UK., Stanton C; Teagasc Food Research Centre, Moorepark, Fermoy, Ireland., Smyth TJ; Department of Life Science, Institute of Technology Sligo, Sligo, Ireland., Soler-Vila A; Irish Seaweed Research Group, Ryan Institute for Environmental, Marine and Energy Research, National University of Ireland, Galway, University Road, Galway, Ireland., Rea MC; Teagasc Food Research Centre, Moorepark, Fermoy, Ireland., Ross PR; Teagasc Food Research Centre, Moorepark, Fermoy, Ireland.; APC Microbiome Institute, University College Cork, Cork, Ireland., Cherry P; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine, BT52 1SA, UK.; APC Microbiome Institute, University College Cork, Cork, Ireland., Allsopp PJ; Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Coleraine, BT52 1SA, UK. pj.allsopp@ulster.ac.uk.
Jazyk: angličtina
Zdroj: European journal of nutrition [Eur J Nutr] 2020 Feb; Vol. 59 (1), pp. 309-325. Date of Electronic Publication: 2019 Feb 25.
DOI: 10.1007/s00394-019-01909-6
Abstrakt: Background: Brown seaweeds are known to be a rich source of fiber with the presence of several non-digestible polysaccharides including laminarin, fucoidan and alginate. These individual polysaccharides have previously been shown to favorably alter the gut microbiota composition and activity albeit the effect of the collective brown seaweed fiber component on the microbiota remains to be determined.
Methods: This study investigated the effect of a crude polysaccharide-rich extract obtained from Laminaria digitata (CE) and a depolymerized CE extract (DE) on the gut microbiota composition and metabolism using an in vitro fecal batch culture model though metagenomic compositional analysis using 16S rRNA FLX amplicon pyrosequencing and short-chain fatty acid (SCFA) analysis using GC-FID.
Results: Selective culture analysis showed no significant changes in cultured lactobacilli or bifidobacteria between the CE or DE and the cellulose-negative control at any time point measured (0, 5, 10, 24, 36, 48 h). Following metagenomic analysis, the CE and DE significantly altered the relative abundance of several families including Lachnospiraceae and genera including Streptococcus, Ruminococcus and Parabacteroides of human fecal bacterial populations in comparison to cellulose after 24 h. The concentrations of acetic acid, propionic acid, butyric acid and total SCFA were significantly higher for both the CE and DE compared to cellulose after 10, 24, 36 and 48 h fermentation (p < 0.05). Furthermore, the acetate:propionate ratio was significantly reduced (p < 0.05) for both CD and DE following 24, 36 and 48 h fermentation.
Conclusion: The microbiota-associated metabolic and compositional changes noted provide initial indication of putative beneficial health benefits of L. digitata in vitro; however, research is needed to clarify if L. digitata-derived fiber can favorably alter the gut microbiota and confer health benefits in vivo.
Databáze: MEDLINE
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