β-Hydroxy-β-methylbutyrate Suppresses NF-ĸB Activation and IL-6 Production in TE-1 Cancer Cells.

Autor:	Miyake S; Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan s-miyake@mw.kawasaki-m.ac.jp., Ogo A; Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan., Kubota H; Department of Digestive Surgery, Kawasaki Medical School, Kurashiki, Japan., Teramoto F; Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan., Hirai T; Department of Doctoral Program in Health Science, Graduate School of Health Science and Technology, Kawasaki University of Medical Welfare, Kurashiki, Japan.
Jazyk:	angličtina
Zdroj:	In vivo (Athens, Greece) [In Vivo] 2019 Mar-Apr; Vol. 33 (2), pp. 353-358.
DOI:	10.21873/invivo.11481
Abstrakt:	Background/aim: Stress reactions, especially those related to surgery, cause poor convalescence of cancer patients. β-Hydroxyβ-methylbutyrate (HMB) is known to regulate excessive inflammation in the body. The objective of this work was to investigate the capacity of HMB to suppress activation of nuclear factor-kappa B (NF-ĸB) and production of interleukin-6 (IL-6) in a human esophageal squamous cell carcinoma cell line (TE-1). Materials and Methods: Cell proliferation was measured using the water-soluble tetrazolium-1 method, while tumor necrosis factor alpha (TNFα)-induced IL-6 production was measured using an enzyme-linked immunosorbent assay (ELISA) assay. Nuclear translocation of NF-ĸB was detected by immunofluorescence staining. Results: HMB did not affect cell proliferation. However, HMB suppressed the TNFα-induced increase in IL-6 production in TE-1 cells by inhibiting NF-ĸB activation. Conclusion: HMB did not influence TE-1 cell proliferation, but inhibited activation of NF-ĸB and IL-6 production. This result may be useful for improving excessive stress reactions during and after surgery. (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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