| Autor: |
Sorokin DY; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia.; 2Department of Biotechnology, TU Delft, Delft, The Netherlands., Khijniak TV; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Elcheninov AG; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Toshchakov SV; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Kostrikina NA; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia., Bale NJ; 3Department of Marine Microbiology and Biogeochemistry, NIOZ Netherlands Institute for Sea Research, and Utrecht University, Utrecht, The Netherlands., Sinninghe Damsté JS; 3Department of Marine Microbiology and Biogeochemistry, NIOZ Netherlands Institute for Sea Research, and Utrecht University, Utrecht, The Netherlands.; 4Department of Earth Sciences - Geochemistry, Faculty of Geosciences, Utrecht University, Utrecht, The Netherlands., Kublanov IV; 1Winogradsky Institute of Microbiology, Research Centre of Biotechnology, Russian Academy of Sciences, Moscow, Russia. |
| Abstrakt: |
An extremely halophilic euryarchaeon, strain HArcel1 T , was enriched and isolated in pure culture from the surface brines and sediments of hypersaline athalassic lakes in the Kulunda Steppe (Altai region, Russia) using amorphous cellulose as the growth substrate. The colonies of HArcel1 T are pale-orange, and form large zones of cellulose hydrolysis around them. The cells are non-motile cocci of variable size with a thin monolayer cell wall. The isolate is an obligate aerobic heterotroph capable of growth with only three substrates: various forms of insoluble cellulose, xylan and cellobiose. Strain HArcel1 T is an extremely halophilic neutrophile, growing within the salinity range from 2.5 to 5 M NaCl (optimum at 3.5-4 M). The core archaeal lipids are dominated by C20-C20 and C25-C20 dialkyl glycerol ethers, in approximately 6:1 proportion. The 16S rRNA and rpoB' gene analysis indicated that HArcel1 T forms a separate lineage within the family Haloarculaceae, order Halobacteriales, with the genera Halorhabdus and Halopricus as closest relatives. On the basis of the unique phenotypic properties and distinct phylogeny of the 16S rRNA and rpoB' genes, it is suggested that strain HArcel1 T is classified into a new genus and species Halococcoides cellulosivorans gen. nov., sp. nov. (JCM 31941 T =UNIQEM U975 T ). |
