Platelet lysates from aged donors promote human tenocyte proliferation and migration in a concentration-dependent manner.

Autor:	Berger DR; Interventional Orthopedics Foundation, Broomfield, Colorado, USA., Centeno CJ; Centeno-Schultz Clinic, Broomfield, Colorado, USA; Board Chairman, Interventional Orthopedics Foundation, Broomfield, Colorado, USA; Chief Medical Officer, Regenexx, LLC, Broomfield, Colorado, USA., Steinmetz NJ; Regenexx, LLC, Broomfield, Colorado, USA.
Jazyk:	angličtina
Zdroj:	Bone & joint research [Bone Joint Res] 2019 Feb 02; Vol. 8 (1), pp. 32-40. Date of Electronic Publication: 2019 Feb 02 (Print Publication: 2019).
DOI:	10.1302/2046-3758.81.BJR-2018-0164.R1
Abstrakt:	Objectives: Platelet-rich plasma (PRP) is being used increasingly often in the clinical setting to treat tendon-related pathologies. Yet the optimal PRP preparations to promote tendon healing in different patient populations are poorly defined. Here, we sought to determine whether increasing the concentration of platelet-derived proteins within a derivative of PRP, platelet lysate (PL), enhances tenocyte proliferation and migration in vitro , and whether the mitogenic properties of PL change with donor age. Methods: Concentrated PLs from both young (< 50 years) and aged (> 50 years) donors were prepared by exposing pooled PRP to a series of freeze-thaw cycles followed by dilution in plasma, and the levels of several platelet-derived proteins were measured using multiplex immunoassay technology. Human tenocytes were cultured with PLs to simulate a clinically relevant PRP treatment range, and cell growth and migration were assessed using DNA quantitation and gap closure assays, respectively. Results: Platelet-derived protein levels increased alongside higher PL concentrations, and PLs from both age groups improved tenocyte proliferation relative to control conditions. However, PLs from aged donors yielded a dose-response relationship in tenocyte behaviour, with higher PL concentrations resulting in increased tenocyte proliferation and migration. Conversely, no significant differences in tenocyte behaviour were detected when increasing the concentration of PLs from younger donors. Conclusion: Higher PL concentrations, when prepared from the PRP of aged but not young donors, were more effective than lower PL concentrations at promoting tenocyte proliferation and migration in vitro . Cite this article : D. R. Berger, C. J. Centeno, N. J. Steinmetz. Platelet lysates from aged donors promote human tenocyte proliferation and migration in a concentration-dependent manner. Bone Joint Res 2019;8:32-40. DOI: 10.1302/2046-3758.81.BJR-2018-0164.R1. Competing Interests: ICMJE COI statement: D. Berger reports the use of laboratory equipment provided by Regenexx, LLC, as well as funding from John C. Malone in the form of a gift in support of the Interventional Orthopedic Foundation’s initiatives in regenerative medicine. N. J. Steinmetz reports consultancy fees, lecture payments, payment for the development of educational presentations, and payment for contributing to the creation of the manuscript, all from Interventional Orthopedic Foundation.
Databáze:	MEDLINE
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