| Autor: |
Wang LC; Department of Marine Biotechnology and Resources, National Sun Yat-Sen University; marknjoy@umd.edu., Wagner J; Department of Cell Biology and Molecular Genetics, University of Maryland., Capino A; Department of Cell Biology and Molecular Genetics, University of Maryland., Nesbit E; Department of Cell Biology and Molecular Genetics, University of Maryland., Song W; Department of Cell Biology and Molecular Genetics, University of Maryland., Stein DC; Department of Cell Biology and Molecular Genetics, University of Maryland. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2019 Feb 08 (144). Date of Electronic Publication: 2019 Feb 08. |
| DOI: |
10.3791/58978 |
| Abstrakt: |
The emergence of antibiotic resistant Neisseria gonorrhoeae (GC) is a worldwide health threat and highlights the need to identify individuals who fail treatment. This Gram-negative bacterium causes gonorrhea exclusively in humans. During infection, it is able to form aggregates and/or biofilms. The minimum inhibitory concentration (MIC) test is used for to determine susceptibility to antibiotics and to define appropriate treatment. However, the mechanism of the eradication in vivo and its relationship to laboratory results are not known. A method that examines how GC aggregation affects antibiotic susceptibility and shows the relationship between aggregate size and antibiotic susceptibility was developed. When GC aggregate, they are more resistant to antibiotic killing, with bacteria in the center surviving ceftriaxone treatment better than those in the periphery. The data indicate that N. gonorrhoeae aggregation can reduce its susceptibility to ceftriaxone, which is not reflected using the standard agar plate-based MIC methods. The method used in this study will allow researchers to test bacterial susceptibility under clinically relevant conditions. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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