Simultaneous determination of twelve dyes in meat products: Development and validation of an analytical method based on HPLC-UV-diode array detection.

Autor: Iammarino M; National Reference Center for the Detection of Radioactivity in Feed and Foodstuff, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: marco.iammarino@tin.it., Mentana A; Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: annalisa.mentana@unifg.it., Centonze D; Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: diego.centonze@unifg.it., Palermo C; Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: carmen.palermo@unifg.it., Mangiacotti M; National Reference Center for the Detection of Radioactivity in Feed and Foodstuff, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: michele.mangiacotti@izspb.it., Chiaravalle AE; National Reference Center for the Detection of Radioactivity in Feed and Foodstuff, Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Via Manfredonia 20, 71121 Foggia, Italy. Electronic address: eugenio.chiaravalle@izspb.it.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2019 Jul 01; Vol. 285, pp. 1-9. Date of Electronic Publication: 2019 Jan 30.
DOI: 10.1016/j.foodchem.2019.01.133
Abstrakt: The use of food dyes in meat is subject to regulations, due to food safety concerns. A reliable method for the determination of 12 food dyes (Amaranth, Ponceau 4R, Carmine, Ponceau SX, Ponceau 3R, Allura Red AC, Carmoisine, Erythrosine, Sudan I, Sudan II, Sudan III and Sudan IV) in meat products using high performance liquid chromatography coupled to UV-diode array detection was developed, optimized and fully validated. The extraction was accomplished using acetonitrile, methanol, water, ammonia, 50:40:9:1 (v/v/v/v) as the solvent, and an ultrasonic bath. Chromatographic separation was achieved using a C 18 RP column and samples eluted with a gradient acetate-acetonitrile mobile phase. Good analytical performance was obtained, in terms of selectivity, sensitivity, accuracy and ruggedness. Both method precision (CV% range: 6.2%-18.0%) and recovery (range: 86.4%-105.0%) complied with Decision 657/2002/EC, suggesting the procedure could be applied successfully for analyses of meat products in the European Union.
(Copyright © 2019 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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