Transcriptomic profiles of retinal ganglion cells are defined by the magnitude of intraocular pressure elevation in adult mice.

Autor: Park YH; Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, 77030, United States., Snook JD; Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, 77030, United States., Ostrin EJ; Pulmonary Medicine, The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, United States., Kim S; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, 77030, United States., Chen R; Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, 77030, United States.; Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, 77030, United States., Frankfort BJ; Department of Ophthalmology, Baylor College of Medicine, Houston, Texas, 77030, United States. benjamin.frankfort@bcm.edu.; Department of Neuroscience, Baylor College of Medicine, Houston, Texas, 77030, United States. benjamin.frankfort@bcm.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2019 Feb 22; Vol. 9 (1), pp. 2594. Date of Electronic Publication: 2019 Feb 22.
DOI: 10.1038/s41598-019-39141-1
Abstrakt: Elevated intraocular pressure (IOP) is the major risk factor for glaucoma, a sight threatening disease of retinal ganglion cells (RGCs) and their axons. Despite the central importance of IOP, details of the impact of IOP elevation on RGC gene expression remain elusive. We developed a 4-step immunopanning protocol to extract adult mouse RGCs with high fidelity and used it to isolate RGCs from wild type mice exposed to 2 weeks of IOP elevation generated by the microbead model. IOP was elevated to 2 distinct levels which were defined as Mild (IOP increase >1 mmHg and <4 mmHg) and Moderate (IOP increase ≥4 mmHg). RNA sequencing was used to compare the transcriptional environment at each IOP level. Differentially expressed genes were markedly different between the 2 groups, and pathway analysis revealed frequently opposed responses between the IOP levels. These results suggest that the magnitude of IOP elevation has a critical impact on RGC transcriptional changes. Furthermore, it is possible that IOP-based set points exist within RGCs to impact the direction of transcriptional change. It is possible that this improved understanding of changes in RGC gene expression can ultimately lead to novel diagnostics and therapeutics for glaucoma.
Databáze: MEDLINE
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