Examination of OPG, RANK, RANKL and HIF1A polymorphisms in temporomandibular joint ankylosis patients.

Autor: Corso PFCL; School of Health Science, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81-280-330, Brazil. Electronic address: paolafcorso@gmail.com., Meger MN; School of Health Science, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81-280-330, Brazil. Electronic address: michellemeger@hotmail.com., Petean IBF; Department of Restorative Dentistry, School of Dentistry of University of São Paulo, Avenida do Café, Campus da USP, Ribeirão Preto, SP, 14040-904, Brazil. Electronic address: igor.petean@usp.br., Souza JF; Department of Stomatology, School of Dentistry of Federal University of Paraná, 632 Prefeito Lothario Meissner Avenue, Jardim Botânico, Curitiba, PR, 80210170, Brazil. Electronic address: julianafeltrin1@gmail.com., Brancher JA; School of Health Science, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81-280-330, Brazil. Electronic address: brancher@up.edu.br., da Silva LAB; Department of Pediatric Dentistry, School of Dentistry of University of São Paulo, Avenida do Café, Campus da USP, Ribeirão Preto, SP, 14040-904, Brazil. Electronic address: lea@forp.usp.br., Rebelatto NLB; Department of Stomatology, School of Dentistry of Federal University of Paraná, 632 Prefeito Lothario Meissner Avenue, Jardim Botânico, Curitiba, PR, 80210170, Brazil. Electronic address: rebelato@ufpr.br., Kluppel LE; Department of Stomatology, School of Dentistry of Federal University of Paraná, 632 Prefeito Lothario Meissner Avenue, Jardim Botânico, Curitiba, PR, 80210170, Brazil. Electronic address: lekluppel@hotmail.com., Sousa-Neto MD; Department of Restorative Dentistry, School of Dentistry of University of São Paulo, Avenida do Café, Campus da USP, Ribeirão Preto, SP, 14040-904, Brazil. Electronic address: sousanet@forp.usp.br., Küchler EC; School of Health Science, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81-280-330, Brazil; Department of Pediatric Dentistry, School of Dentistry of University of São Paulo, Avenida do Café, Campus da USP, Ribeirão Preto, SP, 14040-904, Brazil. Electronic address: erikacalvano@gmail.com., Scariot R; School of Health Science, Positivo University, 5300 Professor Pedro Viriato Parigot de Souza Street, Campo Comprido, Curitiba, PR, 81-280-330, Brazil; Department of Stomatology, School of Dentistry of Federal University of Paraná, 632 Prefeito Lothario Meissner Avenue, Jardim Botânico, Curitiba, PR, 80210170, Brazil. Electronic address: rafaela_scariot@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of cranio-maxillo-facial surgery : official publication of the European Association for Cranio-Maxillo-Facial Surgery [J Craniomaxillofac Surg] 2019 May; Vol. 47 (5), pp. 766-770. Date of Electronic Publication: 2019 Jan 21.
DOI: 10.1016/j.jcms.2019.01.024
Abstrakt: Purpose: To evaluate the association between polymorphisms in genes that regulate bone metabolism, such as OPG, RANK, RANKL, and HIF1A, in patients with temporomandibular joint (TMJ) ankylosis.
Methods: The sample consisted of 181 individuals, the study included 17 individuals with TMJ ankylosis and 164 controls. DNA was extracted from buccal epithelial cells. The genotyping of genetic polymorphisms in OPG (rs2073618), RANK (rs3826620), RANKL (rs9594738), and HIF1A (rs2301113 and rs2057482) was performed by real-time PCR using TaqMan™ technology (Applied Biosystems). The data were subjected to statistical analysis with a level of significance of 0.05.
Results: The OPG (rs2073618) polymorphism was associated with TMJ ankylosis, both in the additive model and in the dominant model (p < 0.05). In the additive model, when the individuals carried the CC genotype, they presented as 10.80 times more likely to develop the condition (p = 0.03). In the dominant model, individuals that carried at least one C allele were 5.76 times more likely to have TMJ ankylosis, than those with the G allele (p = 0.01).
Conclusion: The polymorphism rs2073618 of OPG is a possible marker that is associated with the risk of manifestation of TMJ ankylosis.
(Copyright © 2019 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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