| Autor: |
Bernáldez-Sarabia J; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. jbernald@cicese.edu.mx., Figueroa-Montiel A; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. figuerm@cicese.edu.mx., Dueñas S; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. sduenas@cicese.edu.mx., Cervantes-Luévano K; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. kecervanlu@gmail.com., Beltrán JA; Departamento de Ciencias Computacionales, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. abeltran@cicese.edu.mx., Ortiz E; Departamento de Medicina Molecular y Bioprocesos, IBT, UNAM, Cuernavaca, Morelos, C.P. 62210, Mexico. erne@ibt.unam.mx., Jiménez S; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. mjimenez@cicese.edu.mx., Possani LD; Departamento de Medicina Molecular y Bioprocesos, IBT, UNAM, Cuernavaca, Morelos, C.P. 62210, Mexico. possani@ibt.unam.mx., Paniagua-Solís JF; Teraclon IDF, S.L., Parque Tecnológico de Madrid, Tres Cantos, Madrid, C.P. 28760, Espana. jpaniagua@teraclon.com., Gonzalez-Canudas J; Laboratorio Silanes, S.A. de C.V., Ciudad de México, C.P. 11000, Mexico. jogonzalez@silanes.com.mx., Licea-Navarro A; Departamento de Innovación Biomédica, CICESE, Carretera Ensenada-Tijuana 3918, Ensenada, BC C.P. 22860, Mexico. alicea@cicese.mx. |
| Abstrakt: |
Californiconus californicus , previously named Conus californicus , has always been considered a unique species within cone snails, because of its molecular, toxicological and morphological singularities; including the wide range of its diet, since it is capable of preying indifferently on fish, snails, octopus, shrimps, and worms. We report here a new cysteine pattern conotoxin assigned to the O1-superfamily capable of inhibiting the growth of Mycobacterium tuberculosis (Mtb). The conotoxin was tested on a pathogen reference strain (H37Rv) and multidrug-resistant strains, having an inhibition effect on growth with a minimal inhibitory concentration (MIC) range of 3.52⁻0.22 μM, similar concentrations to drugs used in clinics. The peptide was purified from the venom using reverse phase high-performance liquid chromatography (RP-HPLC), a partial sequence was constructed by Edman degradation, completed by RACE and confirmed with venom gland transcriptome. The 32-mer peptide containing eight cysteine residues was named O1_cal29b, according to the current nomenclature for this type of molecule. Moreover, transcriptomic analysis of O-superfamily toxins present in the venom gland of the snail allowed us to assign several signal peptides to O2 and O3 superfamilies not described before in C. californicus , with new conotoxins frameworks. |
