Hierarchical microchannel architecture in chitosan/bioactive glass scaffolds via electrophoretic deposition positive-replica.

Autor: Ghalayani Esfahani A; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy., Soleimanzade M; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy., Campiglio CE; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy.; Local Unit Politecnico di Milano, INSTM National Interuniversity Consortium of Materials Science and Technology, Florence, Italy., Federici A; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy.; Local Unit Politecnico di Milano, INSTM National Interuniversity Consortium of Materials Science and Technology, Florence, Italy., Altomare L; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy., Draghi L; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy.; Local Unit Politecnico di Milano, INSTM National Interuniversity Consortium of Materials Science and Technology, Florence, Italy., Boccaccini AR; Institute of Biomaterials, University of Erlangen-Nuremberg, 91058 Erlangen, Germany., De Nardo L; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, Milan, Italy.; Local Unit Politecnico di Milano, INSTM National Interuniversity Consortium of Materials Science and Technology, Florence, Italy.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2019 Jul; Vol. 107 (7), pp. 1455-1465. Date of Electronic Publication: 2019 Mar 21.
DOI: 10.1002/jbm.a.36660
Abstrakt: One of the main challenges in the design of scaffolds for cortical bone regeneration is mimicking the highly oriented, hierarchical structure of the native tissue in an efficient, simple, and consistent way. As a possible solution to this challenge, positive replica based on electrophoretic deposition (EPD) was here evaluated as a technique to produce organic/inorganic scaffolds with oriented micro-porosities mimicking Haversian canals diameter and spacing. Two different sizes of 45S5 bioactive glass (BG) powders were chosen as inclusions and loaded in a chitosan matrix via EPD on micro-patterned cathodes. Self-standing chitosan scaffolds, with a homogeneous dispersion of BG particles and regularly-oriented micro-channels (ϕ = 380 ± 50 μm, inter-channel spacing = 600 ± 40 μm), were obtained. In vitro analysis in simulated body fluid (SBF) revealed the ability to induce a deposition of a homogenous layer of hydroxyapatite (HA), with Ca/P nucleation reactions appearing kinetically favored by smaller BG particles. Cell interaction with hybrid scaffolds was evaluated in vitro with bone osteosarcoma cells (SAOS-2). The osteoconductive potential of EPD structures was assessed by evaluating cells proliferation, viability and scaffold colonization. Results indicate that EPD is a simple yet extremely effective technique to prepare composite micro-patterned structures and can represent a platform for the development of a new generation of bone scaffolds. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2019.
(© 2019 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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