Global gene expression changes reflecting pleiotropic effects of Irpex lacteus induced by low--intensity electromagnetic field.

Autor: Sun L; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China., Li X; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China., Ma H; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China., He R; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China., Donkor PO; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu, China.
Jazyk: angličtina
Zdroj: Bioelectromagnetics [Bioelectromagnetics] 2019 Feb; Vol. 40 (2), pp. 104-117. Date of Electronic Publication: 2019 Feb 20.
DOI: 10.1002/bem.22171
Abstrakt: A polysaccharide of Irpex lacteus, a white-rot fungus with lignocellulose-degrading activities, has been used as a commercial medicine for nephritis treatment. Previously, a low-intensity electromagnetic field (LI-EMF) was found to increase the biomass and polysaccharide content of Irpex lacteus and induce twists on the cell surface. In this study, RNA-sequencing (RNA-seq) technology was used to analyze the underlying mechanism of LI-EMF's influence on Irpex lacteus. We identified 3268, 1377, and 941 differentially expressed genes (DEGs) in the LI-EMF-treated samples at recovery times of 0 h, 3 h, and 6 h, respectively, indicating a significant decline in the influence of the LI-EMF treatment on Irpex lacteus with the passage of recovery time. Moreover, 30 upregulated and 14 downregulated DEGs overlapped in the LI-EMF-treated samples at the recovery times of 0 h, 3 h, and 6 h, implying the important lasting effects of LI-EMF. The reliability of the RNA-seq data were validated by quantitative real-time PCR (qRT-PCR). The DEGs related to transcription factors, cell proliferation, cell wall, membrane components, amino acid biosynthesis and metabolism, and polysaccharide biosynthesis and metabolism were significantly enriched in the LI-EMF-treated samples. The experiments confirmed that the LI-EMF treatment significantly increased the content of amino acids with a considerable increase in the content of essential amino acids. Therefore, the global gene expression changes explained the pleiotropic effects of Irpex lacteus induced by the LI-EMF treatment. These findings provide the requisite data for the appropriate design and application of LI-EMF in the fermentation of microorganisms to increase production. Bioelectromagnetics. 40:104-117, 2019. © 2019 Bioelectromagnetics Society.
(© 2019 Bioelectromagnetics Society.)
Databáze: MEDLINE
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