| Autor: |
Benatrehina PA; Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University, College of Pharmacy, Columbus, OH, USA., Chen WL; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA., Czarnecki AA; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA., Kurina S; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA., Chai HB; Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University, College of Pharmacy, Columbus, OH, USA., Lantvit DD; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA., Ninh TN; Institute of Ecology and Biological Resources, Vietnam Academy of Science and Technology Hanoi, Hanoi, Vietnam., Zhang X; Center for Biostatistics, The Ohio State University, Columbus, OH, USA., Soejarto DD; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA.; John G. Searle Herbarium of the Field Museum of Natural History, Chicago, IL, USA., Burdette JE; College of Pharmacy, University of Illinois at Chicago, Chicago, IL, USA., Kinghorn AD; Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University, College of Pharmacy, Columbus, OH, USA., Rakotondraibe LH; Division of Medicinal Chemistry and Pharmacognosy, The Ohio State University, College of Pharmacy, Columbus, OH, USA. rakotondraibe.1@osu.edu. |
| Abstrakt: |
Bioactivity-guided phytochemical investigation of Podocarpus neriifolius D. Don. (Podocarpaceae) has led to the isolation of one new (2) and three known (1, 3, and 4) B-type podolactones, along with three totarane-type diterpenes (5-7). Their structures were determined by interpretation of High Resolution ElectroSpray Ionization Mass Spectrometry (HRESIMS) and 1D and 2D NMR data, and comparison with the values reported in the literature. The structure of compound 1, previously identified as 3-deoxy-2α-hydroxynagilactone E (8), was revised as its 2β-epimer, which has been reported recently as a new compound. All of the isolates were evaluated for their antiproliferative activity against a panel of four human cancer cell lines, namely, ovarian (OVCAR3), breast (MDA-MB-231), colon (HT-29), and melanoma (MDA-MB-435), and compounds 1 and 3 were found to be cytotoxic with IC 50 values in the low micromolar range for most of the cell lines used. The major compound, inumakilactone A (3), was further tested in vivo using the HT-29, MDA-MB-435, and OVCAR3 cells in a murine hollow fiber model, for the first time. |
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