Computationally Optimizing the Compliance of Multilayered Biomimetic Tissue Engineered Vascular Grafts.

Autor:	Tamimi EA; Department of Bioengineering,University of Pittsburgh,Pittsburgh, PA 15213e-mail: ehab.t@pitt.edu., Ardila DC; Department of Bioengineering,University of Pittsburgh,Pittsburgh, PA 15213e-mail: cata.ardila28@pitt.edu., Ensley BD; Protein Genomics, Inc,Sedona, AZ 86336e-mail: burt@burtensley.com., Kellar RS; Center for Bioengineering Innovation,Northern Arizona University,Flagstaff, AZ 86011;Department of Mechanical Engineering,Northern Arizona University,Flagstaff, AZ 86011;Department of Biological Sciences,Northern Arizona University,Flagstaff, AZ 86011e-mail: robert.kellar@nau.edu., Vande Geest JP; Mem. ASMEDepartment of Bioengineering,University of Pittsburgh,Pittsburgh, PA 15213;McGowan Institute for Regenerative Medicine,300 Technology Drive,Pittsburgh, PA 15219e-mail: jpv20@pitt.edu.
Jazyk:	angličtina
Zdroj:	Journal of biomechanical engineering [J Biomech Eng] 2019 Jun 01; Vol. 141 (6).
DOI:	10.1115/1.4042902
Abstrakt:	Coronary artery bypass grafts used to treat coronary artery disease (CAD) often fail due to compliance mismatch. In this study, we have developed an experimental/computational approach to fabricate an acellular biomimetic hybrid tissue engineered vascular graft (TEVG) composed of alternating layers of electrospun porcine gelatin/polycaprolactone (PCL) and human tropoelastin/PCL blends with the goal of compliance-matching to rat abdominal aorta, while maintaining specific geometrical constraints. Polymeric blends at three different gelatin:PCL (G:PCL) and tropoelastin:PCL (T:PCL) ratios (80:20, 50:50, and 20:80) were mechanically characterized. The stress-strain data were used to develop predictive models, which were used as part of an optimization scheme that was implemented to determine the ratios of G:PCL and T:PCL and the thickness of the individual layers within a TEVG that would compliance match a target compliance value. The hypocompliant, isocompliant, and hypercompliant grafts had target compliance values of 0.000256, 0.000568, and 0.000880 mmHg-1, respectively. Experimental validation of the optimization demonstrated that the hypercompliant and isocompliant grafts were not statistically significant from their respective target compliance values (p-value = 0.37 and 0.89, respectively). The experimental compliance values of the hypocompliant graft were statistically significant than their target compliance value (p-value = 0.047). We have successfully demonstrated a design optimization scheme that can be used to fabricate multilayered and biomimetic vascular grafts with targeted geometry and compliance. (Copyright © 2019 by ASME.)
Databáze:	MEDLINE
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