| Autor: |
Vendramin N; National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark. niven@aqua.dtu.dk., Kannimuthu D; Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway., Olsen AB; Norwegian Veterinary Institute, Bergen, Norway., Cuenca A; National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark., Teige LH; Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway., Wessel Ø; Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway., Iburg TM; National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark., Dahle MK; Norwegian Veterinary Institute, Oslo, Norway., Rimstad E; Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway., Olesen NJ; National Institute of Aquatic Resources, Technical University of Denmark, Kongens Lyngby, Denmark. |
| Abstrakt: |
Piscine orthoreovirus (PRV) mediated diseases have emerged throughout salmonid aquaculture. Three PRV subtypes are currently reported as causative agents of or in association with diseases in different salmonid species. PRV-1 causes heart and skeletal muscle inflammation (HSMI) in Atlantic salmon (Salmo salar) and is associated with jaundice syndrome in farmed chinook salmon (Oncorhynchus tshawytscha). PRV-2 causes erythrocytic inclusion body syndrome (EIBS) in coho salmon in Japan. PRV-3 has recently been associated with a disease in rainbow trout (Oncorhynchus mykiss) characterized by anaemia, heart and red muscle pathology; to jaundice syndrome in coho salmon (Oncorhynchus kisutch). In this study, we conducted a 10-week long experimental infection trial in rainbow trout with purified PRV-3 particles to assess the causal relationship between the virus and development of heart inflammation. The monitoring the PRV-3 load in heart and spleen by RT-qPCR shows a progressive increase of viral RNA to a peak, followed by clearance without a measurable change in haematocrit. The development of characteristic cardiac histopathological findings occurred in the late phase of the trial and was associated with increased expression of CD8+, indicating cytotoxic T cell proliferation. The findings indicate that, under these experimental conditions, PRV-3 infection in rainbow trout act similarly to PRV-1 infection in Atlantic salmon with regards to immunological responses and development of heart pathology, but not in the ability to establish a persistent infection. |
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