In vitro osteogenic differentiation potential of the human induced pluripotent stem cells augments when grown on Graphene oxide-modified nanofibers.

Autor: Saburi E; Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Islami M; Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran., Hosseinzadeh S; Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Moghadam AS; Bu-Ali Research Institute, Department of Immunogenetics, Mashhad University of Medical Sciences, Mashhad, Iran., Mansour RN; Stem Cell Technology Research Center, Tehran, Iran., Azadian E; Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Joneidi Z; Department of Genetics and Molecular Medicine, Zanjan University of Medical Science, Zanjan, Iran., Nikpoor AR; Immunogenetic and Cell Culture Department, Immunology Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Immunology, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran., Ghadiani MH; Department of Nephrology, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Khodaii Z; Dietary Supplements and Probiotic Research Center, Alborz University of Medical Sciences, Karaj, Iran. Electronic address: Zkhodaii@yahoo.com., Ardeshirylajimi A; Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: ardeshirylajimi@sbmu.ac.ir.
Jazyk: angličtina
Zdroj: Gene [Gene] 2019 May 15; Vol. 696, pp. 72-79. Date of Electronic Publication: 2019 Feb 15.
DOI: 10.1016/j.gene.2019.02.028
Abstrakt: Due to the several limitations that surgeons are faced during bone tissue implantation there are daily increases in introducing new cell-co-polymer composites for use in bone tissue engineering approaches. In this study tried to develop a suitable nanostructured bio-composite for enhancing osteogenic differentiation of the human induced pluripotent stem cells (iPSCs). Polyvinylidene fluoride-Graphene oxide (PVDF-GO) nanofibers was fabricated by electrospinning and then characterized using scanning electron microscope, tensile and viability assays. After that osteogenic differentiation of the iPSCs was investigated in three groups, including PVDF, PVDF-GO and tissue culture plate as a control group. Alkaline phosphatase activity and calcium content of the iPSCs cultured on PVDF-GO were significantly higher than those cultured on other groups. In addition, Runx2, osteocalcin and osteonectin genes were up regulated in iPSCs cultured on PVDF-GO significantly higher than those cells cultured on PVDF and control. Finally, osteocalcin and osteopontin proteins expression evaluated and the results confirmed higher osteoinductivity of the PVDF-GO nanofibers in comparison with the PVDF nanofibers. According to the results, it was demonstrated that PVDF-GO nanofibers have a great osteoinductive potential and taking together iPSCs-PVDF-GO nanofibrous construct can be an appropriate bio-implant to use for bone tissue engineering applications.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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