A novel "turn-on" mitochondria-targeting near-infrared fluorescent probe for H 2 S detection and in living cells imaging.

Autor: Zhao XJ; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China., Li YT; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China., Jiang YR; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: jiangyr@mail.csu.edu.cn., Yang BQ; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China., Liu C; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China., Liu ZH; Department of Pharmaceutical Engineering, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2019 May 15; Vol. 197, pp. 326-333. Date of Electronic Publication: 2019 Jan 12.
DOI: 10.1016/j.talanta.2019.01.042
Abstrakt: Hydrogen sulfide (H 2 S) has been considered to be involved in cytoprotective processes and redox signaling. It is very meaningful to track and analyze it in mitochondria. Herein, we report a novel "turn-on" mitochondria-targeting near-infrared fluorescent probe (Mito-NIR-SH) for detection of H 2 S in living cells, which was designed and synthesized by introducing 2,4-dinitrophenyl as fluorescence quenching group and H 2 S response moiety into Changsha near-infrared fluorophore (CS-OH). The structure of the fluorophore and the probe were characterized by 1 H NMR, 13 C NMR and mass spectrometry. Meanwhile, Mito-NIR-SH could quantitatively detect H 2 S at concentrations ranging from 0 to 30 μM with a detection limit as low as 89.3 nM, showing good chemical stability, fast "turn-on" response, selectively mitochondrial location, as well as high sensitivity and selectivity toward H 2 S. Based on this, it was successfully applied to imaging exogenous and endogenous H 2 S in living HeLa cells via confocal fluorescence microscopy.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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