Natural and synthetic nanopores directing osteogenic differentiation of human stem cells.

Autor: Greiner JF; Department of Cell Biology, Bielefeld University, Bielefeld, Germany., Gottschalk M; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany., Fokin N; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany., Büker B; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany., Kaltschmidt BP; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany., Dreyer A; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany., Vordemvenne T; Department of Trauma and Orthopedic Surgery, Evangelical Hospital Bielefeld, Bielefeld, Germany., Kaltschmidt C; Department of Cell Biology, Bielefeld University, Bielefeld, Germany; Bielefeld Institute for Nanoscience (BINAS), Bielefeld University, Bielefeld, Germany., Hütten A; Thin Films & Physics of Nanostructures, Bielefeld University, Bielefeld, Germany; Bielefeld Institute for Nanoscience (BINAS), Bielefeld University, Bielefeld, Germany., Kaltschmidt B; Department of Cell Biology, Bielefeld University, Bielefeld, Germany; Molecular Neurobiology, Bielefeld University, Bielefeld, Germany; Bielefeld Institute for Nanoscience (BINAS), Bielefeld University, Bielefeld, Germany. Electronic address: barbara.kaltschmidt@uni-bielefeld.de.
Jazyk: angličtina
Zdroj: Nanomedicine : nanotechnology, biology, and medicine [Nanomedicine] 2019 Apr; Vol. 17, pp. 319-328. Date of Electronic Publication: 2019 Feb 14.
DOI: 10.1016/j.nano.2019.01.018
Abstrakt: Bone regeneration is a highly orchestrated process crucial for endogenous healing procedures after accidents, infections or tumor therapy. Changes in surface nanotopography are known to directly affect the formation of osteogenic cell types, although no direct linkage to the endogenous nanotopography of bone was described so far. Here we show the presence of pores of 31.93 ± 0.97 nm diameter on the surface of collagen type I fibers, the organic component of bone, and demonstrate these pores to be sufficient to induce osteogenic differentiation of adult human stem cells. We further applied SiO 2 nanoparticles thermally cross-linked to a nanocomposite to artificially biomimic 31.93 ± 0.97 nm pores, which likewise led to in vitro production of bone mineral by adult human stem cells. Our findings show an endogenous mechanism of directing osteogenic differentiation of adult stem cells by nanotopological cues and provide a direct application using SiO 2 nanocomposites with surface nanotopography biomimicking native bone architecture.
(Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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