| Autor: |
Cattani J; Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany., Braun T; Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany., Drescher M; Department of Chemistry and Konstanz Research School Chemical Biology, University of Konstanz, Konstanz, Germany. Malte.Drescher@uni-konstanz.de. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1948, pp. 247-260. |
| DOI: |
10.1007/978-1-4939-9124-2_19 |
| Abstrakt: |
Electron paramagnetic resonance (EPR) spectroscopy in combination with site-directed spin labeling is ideally suited to study structure, dynamics, and interactions of intrinsically disordered proteins as alpha-synuclein.Here we describe all steps required for a corresponding study: the spin labeling procedure, sample preparation, spectroscopic experimental procedure, and data analysis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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