Isolation of Polygalacturonase-Producing Bacterial Strain from Tomatoes ( Lycopersicon esculentum Mill.).

Autor: Obafemi YD; Department of Biological Sciences, Covenant University, PMB 1023, Ota, Ogun State, Nigeria., Ajayi AA; Department of Biological Sciences, Augustine University, PMB 1010, Epe, Lagos State, Nigeria., Taiwo OS; Department of Biological Sciences, Covenant University, PMB 1023, Ota, Ogun State, Nigeria., Olorunsola SJ; Department of Biological Sciences, Covenant University, PMB 1023, Ota, Ogun State, Nigeria., Isibor PO; Department of Biological Sciences, Covenant University, PMB 1023, Ota, Ogun State, Nigeria.
Jazyk: angličtina
Zdroj: International journal of microbiology [Int J Microbiol] 2019 Jan 15; Vol. 2019, pp. 7505606. Date of Electronic Publication: 2019 Jan 15 (Print Publication: 2019).
DOI: 10.1155/2019/7505606
Abstrakt: Background: Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables. It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process.
Methods: Isolation of polygalacturonase-producing bacterial strain from tomatoes ( Lycopersicon esculentum Mill.) was studied. Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C). They were identified based on their morphological, biochemical, and molecular characteristics. The enzyme produced was partially purified by the ammonium sulphate precipitation method. Molecular weights and optimum conditions for best enzyme activity were obtained by SDS PAGE technique.
Results: Five bacterial isolates resulted after screening. Bacterial strain code B5 showed highest polygalacturonase activity. Optimum conditions for polygalacturonase PEC B5 were maintained at pH 4.5; temperature 35°C; substrate concentration 0.3 mg/ml, and best activity at less than 5 min of heating. The enzyme PEC B5 was found to weigh 65 kDa and 50 kDa for crude and partially purified aliquots, respectively. The result of 16S rRNA gene sequencing revealed bacterial strain code B5 as Enterobacter tabaci NR146667 having 79% similarity with the NCBI GenBank.
Conclusion: Microorganisms should be developed for large-scale production of enzymes in developing countries.
Databáze: MEDLINE
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