Transcriptome dataset of trunk neural crest cells migrating along the ventral pathway of chick embryos.
Autor: | Murko C; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.; Center for Anatomy and Cell Biology, Medical University of Vienna, Vienna, Austria., Vieceli FM; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA., Bronner M; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. |
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Jazyk: | angličtina |
Zdroj: | Data in brief [Data Brief] 2018 Nov 27; Vol. 21, pp. 2547-2553. Date of Electronic Publication: 2018 Nov 27 (Print Publication: 2018). |
DOI: | 10.1016/j.dib.2018.11.109 |
Abstrakt: | We present a transcriptome dataset generated from migratory chick trunk neural crest cells, which are destined to form components of the peripheral nervous system. Using the Sox10E1 enhancer, which specifically labels neural crest cells migrating on the trunk ventral pathway, we performed fluorescence activated cell sorting (FACS) of electroporated embryos to obtain a pure population of these cells for library preparation and Illumina sequencing. The results provide a list of genes that are enriched in the trunk neural crest. To validate the data, we performed in situ hybridization to visualize expression of selected transcripts. |
Databáze: | MEDLINE |
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