| Autor: |
Xie W; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Chen Y; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Wang Y; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Wei L; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Yin C; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Glaser AK; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Fauver ME; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Seibel EJ; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States., Dintzis SM; University of Washington, School of Medicine, Department of Pathology, Seattle, Washington, United States., Vaughan JC; University of Washington, Department of Chemistry, Seattle, Washington, United States., Reder NP; University of Washington, School of Medicine, Department of Pathology, Seattle, Washington, United States., Liu JTC; University of Washington, Department of Mechanical Engineering, Seattle, Washington, United States.; University of Washington, School of Medicine, Department of Pathology, Seattle, Washington, United States. |
| Abstrakt: |
Intraoperative assessment of breast surgical margins will be of value for reducing the rate of re-excision surgeries for lumpectomy patients. While frozen-section histology is used for intraoperative guidance of certain cancers, it provides limited sampling of the margin surface (typically <1 % of the margin) and is inferior to gold-standard histology, especially for fatty tissues that do not freeze well, such as breast specimens. Microscopy with ultraviolet surface excitation (MUSE) is a nondestructive superficial optical-sectioning technique that has the potential to enable rapid, high-resolution examination of excised margin surfaces. Here, a MUSE system is developed with fully automated sample translation to image fresh tissue surfaces over large areas and at multiple levels of defocus, at a rate of ∼5 min / cm2. Surface extraction is used to improve the comprehensiveness of surface imaging, and 3-D deconvolution is used to improve resolution and contrast. In addition, an improved fluorescent analog of conventional H&E staining is developed to label fresh tissues within ∼5 min for MUSE imaging. We compare the image quality of our MUSE system with both frozen-section and conventional H&E histology, demonstrating the feasibility to provide microscopic visualization of breast margin surfaces at speeds that are relevant for intraoperative use. |
