| Autor: |
Passineau MJ; Cardiovascular Institute, Allegheny Health Network; Michael.Passineau@ahn.org., Gallo PH; Cardiovascular Institute, Allegheny Health Network., Williams G; Cardiovascular Institute, Allegheny Health Network., Perez R; Cardiovascular Institute, Allegheny Health Network., Benza RL; Cardiovascular Institute, Allegheny Health Network. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2019 Jan 23 (143). Date of Electronic Publication: 2019 Jan 23. |
| DOI: |
10.3791/58353 |
| Abstrakt: |
A variety of pathologies lead to pulmonary hypertension (PH), which is defined as a mean pulmonary artery pressure exceeding 25 mmHg at rest. To further diagnose and manage PH, patients undergo repeated right heart catheterizations (RHC) wherein a Swan-Ganz catheter is advanced into a branch of the pulmonary artery and a balloon is inflated to wedge the catheter tip. This article illustrates a protocol whereby pulmonary artery endothelial cells (PAECs) may be harvested from the balloon tips of Swan-Ganz catheters after RHC, and purified with an anti- CD146 affinity column technique to purify putative PAECs. These cells might be used to provide an in situ snapshot of the biological state of the pulmonary vasculature endothelium to complement hemodynamic measurements obtained during RHC. Harvested and purified PAECs may be used for either cell culture or for subsequent analytical assays such as flow cytometery. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
