| Autor: |
Marino S; Division Hematology Oncology, Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA. simarino@iu.edu., Bishop RT; Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK., de Ridder D; Department of Oncology and Metabolism, Medical School, University of Sheffield, Sheffield, UK., Delgado-Calle J; Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA., Reagan MR; Center for Molecular Medicine, Maine Medical Centre Research Institute, Scarborough, ME, USA. |
| Abstrakt: |
Co-culture assays are used to study the mutual interaction between cells in vitro. This chapter describes 2D and 3D co-culture systems used to study cell-cell signaling crosstalk between cancer cells and bone marrow adipocytes, osteoblasts, osteoclasts, and osteocytes. The chapter provides a step-by-step guide to the most commonly used cell culture techniques, functional assays, and gene expression. |
