Preconditioning human natural killer cells with chorionic villous mesenchymal stem cells stimulates their expression of inflammatory and anti-tumor molecules.

Autor: Abumaree MH; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia. mohamedabumaree@hotmail.com.; College of Science and Health Professions, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 3660, 11481, Mail Code 3124, Riyadh, Saudi Arabia. mohamedabumaree@hotmail.com., Alshehri NA; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia., Almotery A; College of Applied Medical Sciences, King Saud Bin Abdulaziz University for Health Sciences, P.O. Box 3660, 11481, Mail Code, Riyadh, 3124, Saudi Arabia., Al Subayyil AM; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia., Bahattab E; National Center for Stem Cell Technology, Life Sciences and Environment Research Institute, King Abdulaziz City for Science and Technology, P.O Box 6086, Riyadh, 11442, Saudi Arabia., Abomaray FM; Department of Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, Karolinska Institutet, 14186, Stockholm, Sweden.; Center for Hematology and Regenerative Medicine, Karolinska Institutet, 14186, Stockholm, Sweden., Khatlani T; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia., Kalionis B; Department of Maternal-Fetal Medicine Pregnancy Research Centre, Department of Obstetrics and Gynaecology, Royal Women's Hospital, University of Melbourne, Parkville, Victoria, 3052, Australia., Jawdat D; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia., El-Muzaini MF; Department of Obstetrics and Gynaecology, King Abdulaziz Medical City, Minstry of National Guard Health Affairs, P.O. Box 3660, 11481, Mail Code, Riyadh, 3124, Saudi Arabia., Al Jumah MA; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia., AlAskar AS; Stem Cells and Regenerative Medicine Department, King Abdullah International Medical Research Center, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code 1515, Riyadh, Saudi Arabia.; College of Medicine, King Saud Bin Abdulaziz University for Health Sciences, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 3660, 11481, Mail Code, Riyadh, 3124, Saudi Arabia.; Adult Hematology and Stem Cell Transplantation, King Abdulaziz Medical City, Ministry of National Guard Health Affairs, P.O. Box 22490, 11426, Mail Code, Riyadh, 1515, Saudi Arabia.
Jazyk: angličtina
Zdroj: Stem cell research & therapy [Stem Cell Res Ther] 2019 Feb 06; Vol. 10 (1), pp. 50. Date of Electronic Publication: 2019 Feb 06.
DOI: 10.1186/s13287-019-1153-9
Abstrakt: Background: Mesenchymal stem cells derived from the chorionic villi of human placentae (pMSCs) produce a unique array of mediators that regulate the essential cellular functions of their target cells. These properties make pMSCs attractive candidates for cell-based therapy. Here, we examined the effects of culturing human natural killer (NK) cells with pMSCs on NK cell functions.
Methods: pMSCs were cultured with IL-2-activated and non-activated NK cells. NK cell proliferation and cytolytic activities were monitored. NK cell expression of receptors mediating their cytolytic activity against pMSCs, and the mechanisms underlying this effect on pMSCs, were also investigated.
Results: Our findings show that IL-2-activated NK cells, but not freshly isolated NK cells, efficiently lyse pMSCs and that this response might involve the activating NK cell receptor CD69. Interestingly, although pMSCs expressed HLA class I molecules, they were nevertheless lysed by NK cells, suggesting that HLA class I antigens do not play a significant role in protecting pMSCs from NK cell cytolytic activity. Co-culturing NK cells with pMSCs also inhibited NK cell expression of receptors, including CD69, NKpG2D, CD94, and NKp30, although these co-cultured NK cells were not inhibited in lysing cancer cells in vitro. Importantly, co-cultured NK cells significantly increased their production of molecules with anti-tumor effects.
Conclusions: These findings suggest that pMSCs might have potential applications in cancer therapy.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje