| Autor: |
Caetano Da Silva C; 1 Discipline of Endocrinology, Internal Medicine Department, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil., Kollár T; 2 Department of Aquaculture, Szent István University, Gödöllő, Hungary., Csenki-Bakos Z; 2 Department of Aquaculture, Szent István University, Gödöllő, Hungary., Fernandes BHV; 3 Diretoria Técnica de Apoio ao Ensino e Pesquisa da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil., Horváth Á; 2 Department of Aquaculture, Szent István University, Gödöllő, Hungary., Carvalho LR; 1 Discipline of Endocrinology, Internal Medicine Department, Hospital das Clínicas HCFMUSP, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil. |
| Abstrakt: |
We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200 mM glucose, 40 mM KCl, 30 mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method. |
