Surface Modification of Electrospun Scaffolds for Endothelialization of Tissue-Engineered Vascular Grafts Using Human Cord Blood-Derived Endothelial Cells.

Autor: Ardila DC; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. dca13@pitt.edu., Liou JJ; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. jrl101@pitt.edu., Maestas D; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21231, USA. dmaesta1@jhmi.edu., Slepian MJ; Sarver Heart Center, The University of Arizona, Tucson, AZ 85721, USA. slepian@email.arizona.edu.; The Arizona Center for Accelerated BioMedical Innovation, University of Arizona, Tucson, AZ 85721, USA. slepian@email.arizona.edu.; BIO5 Institute for Biocollaborative Research, The University of Arizona, Tucson, AZ 85721, USA. slepian@email.arizona.edu.; Interventional Cardiology, University of Arizona, Tucson, AZ 85721, USA. slepian@email.arizona.edu., Badowski M; Arizona Health Science Center Biorepository, University of Arizona, Tucson, AZ 85724, USA. badowski@email.arizona.edu., Wagner WR; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. wagnerwr@upmc.edu.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA. wagnerwr@upmc.edu.; Department of Surgery, University of Pittsburgh, Pittsburgh, PA 15219, USA. wagnerwr@upmc.edu., Harris D; Arizona Health Science Center Biorepository, University of Arizona, Tucson, AZ 85724, USA. davidh@email.arizona.edu.; Department of Immunobiology, Arizona Health Science Center Biorepository, University of Arizona, Tucson, AZ 85724, USA. davidh@email.arizona.edu., Vande Geest JP; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA 15219, USA. jpv20@pitt.edu.; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA 15219, USA. jpv20@pitt.edu.; Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15219, USA. jpv20@pitt.edu.
Jazyk: angličtina
Zdroj: Journal of clinical medicine [J Clin Med] 2019 Feb 04; Vol. 8 (2). Date of Electronic Publication: 2019 Feb 04.
DOI: 10.3390/jcm8020185
Abstrakt: Tissue engineering has gained attention as an alternative approach for developing small diameter tissue-engineered vascular grafts intended for bypass surgery, as an option to treat coronary heart disease. To promote the formation of a healthy endothelial cell monolayer in the lumen of the graft, polycaprolactone/gelatin/fibrinogen scaffolds were developed, and the surface was modified using thermoforming and coating with collagen IV and fibronectin. Human cord blood-derived endothelial cells (hCB-ECs) were seeded onto the scaffolds and the important characteristics of a healthy endothelial cell layer were evaluated under static conditions using human umbilical vein endothelial cells as a control. We found that polycaprolactone/gelatin/fibrinogen scaffolds that were thermoformed and coated are the most suitable for endothelial cell growth. hCB-ECs can proliferate, produce endothelial nitric oxide synthase, respond to interleukin 1 beta, and reduce platelet deposition.
Databáze: MEDLINE
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