Gardnerella vaginalis and Prevotella bivia Trigger Distinct and Overlapping Phenotypes in a Mouse Model of Bacterial Vaginosis.

Autor:	Gilbert NM; Department of Obstetrics and Gynecology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center or Reproductive Health Sciences, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center for Women's Infectious Disease Research, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri., Lewis WG; Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center for Women's Infectious Disease Research, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri., Li G; Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center for Women's Infectious Disease Research, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Yangzhou University College of Medicine, Yangzhou, Jiangsu, People's Republic of China., Sojka DK; Department of Medicine, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri., Lubin JB; Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center for Women's Infectious Disease Research, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Department of Biological Sciences, University of Delaware, Newark., Lewis AL; Department of Obstetrics and Gynecology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Department of Molecular Microbiology, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.; Center for Women's Infectious Disease Research, Rheumatology Division, Washington University School of Medicine, St. Louis, Missouri.
Jazyk:	angličtina
Zdroj:	The Journal of infectious diseases [J Infect Dis] 2019 Aug 30; Vol. 220 (7), pp. 1099-1108.
DOI:	10.1093/infdis/jiy704
Abstrakt:	Background: Bacterial vaginosis (BV) is a common imbalance of the vaginal microbiota characterized by overgrowth of diverse Actinobacteria, Firmicutes, and Gram-negative anaerobes. Women with BV are at increased risk of secondary reproductive tract infections and adverse pregnancy outcomes. However, which specific bacteria cause clinical features of BV is unclear. Methods: We previously demonstrated that Gardnerella vaginalis could elicit many BV features in mice. In this study, we established a BV model in which we coinfected mice with G. vaginalis and another species commonly found in women with BV: Prevotella bivia. Results: This coinfection model recapitulates several aspects of human BV, including vaginal sialidase activity (a diagnostic BV feature independently associated with adverse outcomes), epithelial exfoliation, and ascending infection. It is notable that G. vaginalis facilitated uterine infection by P. bivia. Conclusions: Taken together, our model provides a framework for advancing our understanding of the role of individual or combinations of BV-associated bacteria in BV pathogenesis. (© The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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