Evaluation of a viral DNA-protein immunization strategy against African swine fever in domestic pigs.

Autor: Pérez-Núñez D; CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain., Sunwoo SY; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Sánchez EG; CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain., Haley N; Department of Microbiology and Immunology, Arizona College of Osteopathic Medicine, Midwestern University, Glendale, AZ, 85308, USA., García-Belmonte R; CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain., Nogal M; CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain., Morozov I; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Madden D; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Gaudreault NN; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Mur L; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Shivanna V; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA., Richt JA; Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA. Electronic address: jricht@vet.k-state.edu., Revilla Y; CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain. Electronic address: yrevilla@cbm.csic.es.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2019 Feb; Vol. 208, pp. 34-43. Date of Electronic Publication: 2018 Dec 21.
DOI: 10.1016/j.vetimm.2018.11.018
Abstrakt: African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
(Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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