Bioactive exopolysaccharide from Neopestalotiopsis sp. strain SKE15: Production, characterization and optimization.

Autor: Fooladi T; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, 1993893973, Tehran, Iran. Electronic address: t.foladi@alzahra.ac.ir., Soudi MR; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, 1993893973, Tehran, Iran. Electronic address: msoudi@alzahra.ac.ir., Alimadadi N; Department of Microbiology, Faculty of Biological Sciences, Alzahra University, 1993893973, Tehran, Iran., Savedoroudi P; Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, District 1, Daneshjou Boulevard, 1983969411, Tehran, Iran., Heravi MM; Department of Chemistry, School of Science, Alzahra University, 1993893973, Tehran, Iran.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 May 15; Vol. 129, pp. 127-139. Date of Electronic Publication: 2019 Jan 30.
DOI: 10.1016/j.ijbiomac.2019.01.203
Abstrakt: Fungal exopolysaccharides are powerful resources of medicinal applications. Neopestalotiopsis sp. SKE15 was isolated and identified according to phenotypical and genotypical analyses (GenBank Accession No. MG649986). The exopolysaccharide (EPS) was produced by cultivation of mycelia in broth culture and extracted. The production was optimized to 2.02 g/l after selection of agitation, temperature, FeSO 4 and K 2 HPO 4 concentrations as the most influencing factors using Placket-Burman design and then by applying response surface methodology. Analytical Tools showed that the EPS is composed of a polysaccharide (1.5-2.1 × 10 6  Da) and its probable low molecular weight derivatives, in a wide range of chain lengths, among them an oligosaccharide of about 1970 Da was dominant. GC-MS (Gas chromatography-mass spectrometry) analysis revealed the EPS was mainly constructed from d-glucose, sorbitol and D-galactose. The EPS showed antibacterial activity against Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633 and Pseudomonas aeruginosa ATCC 27853. DPPH (1,1-diphenyl-2-picryl-hydrazyl) and hydroxyl radical scavenging activity assays showed strong antioxidant activity of the EPS. A challenge with three different cancerous cell lines showed cytotoxic activity of the EPS at final concentration of 100 and 200 μg/ml. Further investigation on medicinal applications of the biopolymer is promising.
(Copyright © 2019. Published by Elsevier B.V.)
Databáze: MEDLINE
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