Extract of Emblica officinalis enhances the growth of human keratinocytes in culture.

Autor: Yamakami Y; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan., Morino K; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan., Takauji Y; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan., Kasukabe R; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan., Miki K; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan., Hossain MN; Department of Biochemistry, Primeasia University, Dhaka 1213, Bangladesh., Ayusawa D; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan; Ichiban Life Corporation, Naka-ku, Yokohama 231-0048, Japan., Fujii M; Graduate School of Nanobioscience, Yokohama City University, Kanazawa-ku, Yokohama 236-0027, Japan. Electronic address: mifuji@yokohama-cu.ac.jp.
Jazyk: angličtina
Zdroj: Journal of integrative medicine [J Integr Med] 2019 Mar; Vol. 17 (2), pp. 141-146. Date of Electronic Publication: 2019 Jan 12.
DOI: 10.1016/j.joim.2019.01.004
Abstrakt: Objective: Keratinocytes are the predominant cell type in the epidermis and play key roles in epidermal function. Thus, identification of the compounds that regulate the growth of keratinocytes is of importance. Here we searched for such compounds from the herbs used in traditional medicine Ayurveda.
Methods: Human keratinocytes were cultured in the presence or absence of the herbal extracts for 2 weeks; the effect of the extracts on cell growth was determined by staining the cells with Coomassie brilliant blue. To detect the compounds that regulate the growth of keratinocytes, the herbal extracts were subjected to high-performance liquid chromatography (HPLC).
Results: We found that the extract of Emblica officinalis enhanced the growth of keratinocytes in culture. Further, we fractionated the extract of E. officinalis using HPLC and identified the fractions responsible for the enhanced growth of keratinocytes.
Conclusion: The extract of E. officinalis enhanced the growth of human keratinocytes in culture. E. officinalis contains the compounds that would be beneficial for human skin health because enhanced growth of keratinocytes would promote wound healing.
(Copyright © 2019 Shanghai Changhai Hospital. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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