| Autor: |
Saleh KA; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. dr_ksaleh@yahoo.com., Albinhassan TH; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. tahani_hassan1413@hotmail.com., Elbehairi SEI; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. seragalone@gmail.com., Alshehry MA; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. alshehri44@gmail.com., Alfaifi MY; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. almashnawe@hotmail.com., Al-Ghazzawi AM; Department of Chemistry, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. algawazy@kku.edu.sa., Al-Kahtani MA; Department of Biology, Science College, King Khalid University, P.O. Box 9004 Abha, Saudi Arabia. mueaalqahtani@kku.edu.sa., Alasmari ADA; Asser Toxicology Center, King Abduallah Street, 61441, P.O. Box 1988 Abha, Saudi Arabia. azalasmari@gmail.com. |
| Abstrakt: |
Natural products, especially secondary metabolites produced by plants under stressed conditions, are shown to have different pharmacological impacts from one to another. Aeluropus lagopoides is one of the common halophyte plants that survive under stressed conditions, and has been used for healing wounds and as a painkiller. The bioactivity and the chemical composition of this plant have been poorly investigated. Consequently, the chemical components of A. lagopoides leaves were extracted using hexane (nonpolar), ethyl acetate (semi-polar), and n -butanol (polar) to extract the most extensive variety of metabolites. The cytotoxicity and anticancer impact of extracted secondary metabolites were evaluated against breast (MCF-7), colon (HCT-116), and liver (HepG2) cancer cell lines using a SulphoRhodamine-B (SRB) test. Their mechanisms of action were verified by observing the appearance of apoptotic bodies using the fluorescent microscope, while their antiproliferative impacts were evaluated using a flow cytometer. Results revealed that secondary metabolites extracted using hexane and ethyl acetate had the highest cytotoxicity and thus the greatest anticancer activity effect on HepG2 with IC 50 (24.29 ± 0.85 and 11.22 ± 0.679 µg/mL, respectively). On the other hand, flow cytometer results showed that secondary metabolites could inhibit the cell cycle in the G0/G1 phase. To ascertain the chemical composition⁻function relationship, the extracts were analyzed using LC-MS/MS. Accordingly, A. lagopoides hexane and ethyl acetate extracts may contain agents with anticancer potential. |
