Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil.

Autor: de Aquino Diniz LV; Department of Veterinary Medicine, Faculdades Integradas de Ourinhos, Rodovia BR 153, Km 338+420m, Ourinhos, SP, 19909-100, Brazil., Minutti AF; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 km 380, Londrina, PR, 86057-970, Brazil., de Souza Lima Nino B; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 km 380, Londrina, PR, 86057-970, Brazil., Costa LR; Department of Veterinary Medicine, Faculdades Integradas de Ourinhos, Rodovia BR 153, Km 338+420m, Ourinhos, SP, 19909-100, Brazil., Bosculo MRM; Department of Veterinary Medicine, Faculdades Integradas de Ourinhos, Rodovia BR 153, Km 338+420m, Ourinhos, SP, 19909-100, Brazil., de Almeida BFM; Department of Veterinary Medicine, Faculdades Integradas de Ourinhos, Rodovia BR 153, Km 338+420m, Ourinhos, SP, 19909-100, Brazil., Garcia JL; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 km 380, Londrina, PR, 86057-970, Brazil., de Barros LD; Department of Veterinary Medicine, Faculdades Integradas de Ourinhos, Rodovia BR 153, Km 338+420m, Ourinhos, SP, 19909-100, Brazil. daniel_vetuel@hotmail.com.; Department of Preventive Veterinary Medicine, Laboratory of Animal Protozoology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, Pr 445 km 380, Londrina, PR, 86057-970, Brazil. daniel_vetuel@hotmail.com.
Jazyk: angličtina
Zdroj: Tropical animal health and production [Trop Anim Health Prod] 2019 Jul; Vol. 51 (6), pp. 1751-1755. Date of Electronic Publication: 2019 Jan 31.
DOI: 10.1007/s11250-019-01828-y
Abstrakt: Neospora caninum is considered as one of the main causes of reproductive failure in cattle. Vertical transmission is the main route of infection in the bovine host and plays an important role in maintaining the parasite in the herd. Molecular detection of N. caninum is important to determine the occurrence of the disease and to evaluate the genetic diversity of the parasite. The present study aimed at assessing the vertical transmission of N. caninum using molecular techniques to detect the parasite in tissue samples from bovine fetuses collected in a slaughterhouse in the state of São Paulo, Brazil. Seventy fetuses and 70 blood samples from pregnant cows were collected in a slaughtering line. Fresh samples of heart and brain tissue from fetuses were analyzed using molecular assays. Serum samples from fetuses and cows were subjected to an indirect fluorescent antibody test (IFAT) to detect antibodies against N. caninum. Nested PCR targeting the internal transcriber 1 (ITS1) region of the protozoan organism was used in the molecular testing. From the total of fetuses examined, 71.42% were positive for N. caninum by PCR. A higher number of heart samples (47.1%) were positive for the parasite using this technique. Antibodies against the protozoa were detected in 12.9% of serum samples of cows; 2.8% of fetuses were seropositive for this pathogen. Our results show that vertical transmission of N. caninum occurs in cattle from this region of Brazil, and that the use of different diagnostic techniques contributes to successful diagnosis of congenital transmission of the parasite in cattle.
Databáze: MEDLINE
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