Field evaluation of a dual rapid Human Immunodeficiency Virus and treponemal syphilis rapid test in community-based clinics in Los Angeles and New York.

Autor: Stafylis C; David Geffen School of Medicine at UCLA, 10920 Wilshire Blvd, Los Angeles, CA, 90024, USA. Electronic address: chrstafylis@outlook.com., Bristow CC; Division of Infectious Diseases and Global Public Health, Department of Medicine, University of California San Diego, 9500 Gilman Dr 0507, La Jolla, CA 92093, USA., Natoli LJ; AIDS Healthcare Foundation, 1811 N Western Ave, Los Angeles, CA, 90027, USA., Salow KR; AIDS Healthcare Foundation, 1811 N Western Ave, Los Angeles, CA, 90027, USA., Davidson E; AIDS Healthcare Foundation, 1811 N Western Ave, Los Angeles, CA, 90027, USA., Granados Y; AIDS Healthcare Foundation, 1811 N Western Ave, Los Angeles, CA, 90027, USA., McGrath M; AIDS Healthcare Foundation, 1811 N Western Ave, Los Angeles, CA, 90027, USA., Klausner JD; David Geffen School of Medicine at UCLA, 10920 Wilshire Blvd, Los Angeles, CA, 90024, USA.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2019 Apr; Vol. 93 (4), pp. 325-328. Date of Electronic Publication: 2018 Oct 11.
DOI: 10.1016/j.diagmicrobio.2018.10.002
Abstrakt: Introduction: Dual human immunodeficiency virus/syphilis rapid diagnostic devices can play an important role in prevention efforts. The field performance of the INSTI Multiplex HIV-1/HIV-2/Syphilis Antibody Test (Multiplex) was evaluated.
Methods: Fingerstick whole blood was tested using the rapid test. A fourth-generation HIV laboratory assay and treponemal-specific laboratory assays were used as reference. Rapid plasma reagin (RPR) was used to stratify treponemal results. Sensitivity and specificity were calculated.
Results: Overall, 274 patients participated. Sensitivity of the Multiplex for detection of HIV was 98.8% (95% CI, 93.4-100%), and specificity was 100% (95% CI, 98.1-100%). Sensitivity for detection of syphilis antibodies was 56.8% (95% CI, 44.7-68.2%), and specificity was 98.5% (95% CI, 95.7-99.7%). Sensitivity for treponemal antibodies improved with increasing RPR and was 100% (95% CI, 78.2-100%) among samples with RPR titers ≥1:8.
Conclusions: The Multiplex showed excellent performance for detection of HIV antibodies and increasing sensitivity for detection of treponemal antibody with increasing RPR titer.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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