Chitosan/hyaluronic acid multilayer films are biocompatible substrate for Wharton's jelly derived stem cells.

Autor: Dennaoui H; Laboratory of Applied Biotechnology: Biomolecules, Biotherapies and Bioprocesses, AZM Centre for Biotechnology research and its Applications, Doctoral School of Science and Technology, Lebanese University, Tripoli, Lebanon., Chouery E; Medical Genetics Unit, Faculty of medicine, Saint Joseph University (USJ), Beirut, Lebanon., Rammal H; Equipe de Recherche sur les relations Matrice extracellulaire-Cellules (ERRMECe), Institut des Materiaux, Maison International de la Recherche, Universite de Cergy-Pontoise, 95000 Neuville sur Oise, France., Abdel-Razzak Z; Laboratory of Applied Biotechnology: Biomolecules, Biotherapies and Bioprocesses, AZM Centre for Biotechnology research and its Applications, Doctoral School of Science and Technology, Lebanese University, Tripoli, Lebanon., Harmouch C; Laboratory of Applied Biotechnology: Biomolecules, Biotherapies and Bioprocesses, AZM Centre for Biotechnology research and its Applications, Doctoral School of Science and Technology, Lebanese University, Tripoli, Lebanon.
Jazyk: angličtina
Zdroj: Stem cell investigation [Stem Cell Investig] 2018 Dec 20; Vol. 5, pp. 47. Date of Electronic Publication: 2018 Dec 20 (Print Publication: 2018).
DOI: 10.21037/sci.2018.12.02
Abstrakt: Background: Discovery of mesenchymal stem cells (MSCs) in various adult human tissues opened the way to new therapeutic strategies involving tissue engineering from these cells. More recently, vascular substitutes have opened the era of vascular engineering by making replacement vessels from purely biological material. The objective of our study was to create a vascular substitute from MSCs using a multilayer polyelectrolyte film based on natural polymers (Chitosan and Hyaluronic Acid).
Methods: Biocompatibility and cellular behavior were evaluated in this study using MSCs from the Wharton's jelly (WJ) of human umbilical cords. WJ-MSCs adherence was assessed and cells morphology was investigated by Scanning Electron Microscopy (SEM) and actin visualization (Phalloidin).
Results: The number of WJ-MSCs seeded on the (CHI/HA) 10 films was greater than the number of cells seeded on the collagen, as the spectrophotometric measurement showed a large cell proliferation on (CHI/HA) 10 in comparison with collagen. After adhesion, WJ-MSCs showed a fibroblastic morphology on CHI/HA as for control (collagen I). These results were confirmed by cytoskeleton staining.
Conclusions: The biocompatibility of WJ-MSCs and (CHI/HA) 10 showed the possibility to combine the use of WJ-MSCs and (CHI/HA) 10 films in vascular tissue engineering.
Competing Interests: Conflicts of Interest: The authors have no conflicts of interest to declare.
Databáze: MEDLINE
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