Metaproteomics reveals potential mechanisms by which dietary resistant starch supplementation attenuates chronic kidney disease progression in rats.

Autor: Zybailov BL; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Glazko GV; Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Rahmatallah Y; Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Andreyev DS; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., McElroy T; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Karaduta O; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Byrum SD; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Orr L; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Tackett AJ; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.; Proteomics Core Facility, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Mackintosh SG; Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.; Proteomics Core Facility, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Edmondson RD; Proteomics Core Facility, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Kieffer DA; Department of Nutrition, University of California, Davis, CA, United States of America., Martin RJ; Department of Nutrition, University of California, Davis, CA, United States of America., Adams SH; Arkansas Children's Nutrition Center and Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America., Vaziri ND; Division of Nephrology, University of California, Irvine, CA, United States of America., Arthur JM; Division of Nephrology, University of Arkansas for Medical Sciences, Little Rock, AR, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2019 Jan 30; Vol. 14 (1), pp. e0199274. Date of Electronic Publication: 2019 Jan 30 (Print Publication: 2019).
DOI: 10.1371/journal.pone.0199274
Abstrakt: Background: Resistant starch is a prebiotic metabolized by the gut bacteria. It has been shown to attenuate chronic kidney disease (CKD) progression in rats. Previous studies employed taxonomic analysis using 16S rRNA sequencing and untargeted metabolomics profiling. Here we expand these studies by metaproteomics, gaining new insight into the host-microbiome interaction.
Methods: Differences between cecum contents in CKD rats fed a diet containing resistant starch with those fed a diet containing digestible starch were examined by comparative metaproteomics analysis. Taxonomic information was obtained using unique protein sequences. Our methodology results in quantitative data covering both host and bacterial proteins.
Results: 5,834 proteins were quantified, with 947 proteins originating from the host organism. Taxonomic information derived from metaproteomics data surpassed previous 16S RNA analysis, and reached species resolutions for moderately abundant taxonomic groups. In particular, the Ruminococcaceae family becomes well resolved-with butyrate producers and amylolytic species such as R. bromii clearly visible and significantly higher while fibrolytic species such as R. flavefaciens are significantly lower with resistant starch feeding. The observed changes in protein patterns are consistent with fiber-associated improvement in CKD phenotype. Several known host CKD-associated proteins and biomarkers of impaired kidney function were significantly reduced with resistant starch supplementation. Data are available via ProteomeXchange with identifier PXD008845.
Conclusions: Metaproteomics analysis of cecum contents of CKD rats with and without resistant starch supplementation reveals changes within gut microbiota at unprecedented resolution, providing both functional and taxonomic information. Proteins and organisms differentially abundant with RS supplementation point toward a shift from mucin degraders to butyrate producers.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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