Identification of Clinical Immunological Determinants in Asymptomatic VL and Post Kala-azar Dermal Leishmaniasis Patients.

Autor: Singh AK; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., DAS VNR; Dept. of Pathology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Amit A; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Dikhit MR; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Mahantesh V; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Kumar A; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Pandey RK; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Naryan S; Dept. of Microbiology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Singh BK; Dept. of Clinical Medicine, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Pandey K; Dept. of Clinical Medicine, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Verma N; Dept. of Pathology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., DAS P; Dept. of Molecular Biology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India., Bimal S; Dept. of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, India.
Jazyk: angličtina
Zdroj: Iranian journal of parasitology [Iran J Parasitol] 2018 Oct-Dec; Vol. 13 (4), pp. 541-548.
Abstrakt: Background: Visceral Leishmaniasis (VL) caused by protozoa belonging to the genus Leishmania, usually have anthroponotic mode of transmission and is issue of great public health importance in Indian subcontinent. Asymptomatic cases of VL and PKDL are subject of keen interest to find their role in the transmission of VL in epidemic areas. We evaluated the immunological cytokine determinants expressed in most clinical suspects of asymptomatic VL and PKDL (IL-10, IFN-γ, and TNF-α).
Methods: Eighty-four participants were included at RMRIMS, Patna, India in 2016-17 out of which 64 asymptomatic individual positive for rK-39, without sign and symptoms of VL; 15 PKDL patient's with past history of VL and 5 endemic healthy subjects were recruited from VL endemic areas. DAT and quantitative assessment of plasma cytokines was determined from the blood samples collected in a plain and sodium-EDTA vacutainer respectively from the subjects.
Results: The mean level of IL-10 in DAT pos LOW of asymptomatic VL and PKDL was significantly higher than endemic healthy ( P <0.05). The cytokine polarization index (IFN-γ versus IL-10) was significantly low in PKDL cases compared with asymptomatic VL cases in DAT pos LOW titre ( P <0.05). This index was low again but statistically not significant in PKDL than in asymptomatic VL when TNF-α was considered against IL-10. The ratio of IFN-γ: IL-10 and TNF-α: IL-10 was observed decreased both in asymptomatic VL and PKDL cases than in healthy from endemic areas.
Conclusion: Collectively we surmise from our data that asymptomatic VL can also play an important role like PKDL in transmission of VL.
Competing Interests: Conflict of interest The authors declare that there is no conflict of interests.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje