[IMMUNOCHEMICAL ANALYSIS OF RECOMBINANT CHIMERIC POLYPEPTIDE OspC(gar+afz) OF BORRELIA GARINIIANIP B. AFZELI ISOLATES].
| Autor: | Karavaev VS, Oleinikova ES, Azaev MS, Beklemishev AB |
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| Jazyk: | ruština |
| Zdroj: | Zhurnal mikrobiologii, epidemiologii i immunobiologii [Zh Mikrobiol Epidemiol Immunobiol] 2016 May (3), pp. 37-44. |
| Abstrakt: | Aim: Comparative study of antigenic properties of recombinant proteins OsPCgar and OsPCafz and recombinant chimeric polypeptide OspCgar+afrz, that contains amino acid sequences of mature immune dominant OspC proteins of West-Siberian isolates of Borrelia garinii (OspCgar) and B. afzelii (OspCafz), and evaluation of possibility of their use as antigens during creation of test-systems for serodiagnostics of Lyme borreliosis (LB) on the territory of Western Siberia. Materials and Methods: Recombinant chimeric polypeptide OSpCgar+af, and recombinant mature proteins OSPCgar and OspCafz, obtained by expression of the corresponding genes in Escherichia coli cells; purified by affinity chromatography in Ni-NTA-sepharose CL-6B and studied by EIA method for the ability to bind antibodies from sera of LB patients. Results: A difference in sensitiv- ity of determination by EIA method of specific IgM and IgG against borreliae in blood sera of LB patients with localized stage of the disease during use of OspCgar,'OSPCafz and OspCgar+afz chimera as antigens was shown. Chimeric antigen OSPCgar+afz was established to show higher antigenic activity compared with each of the OspCgar or OSPCafz antigens separately. Conclusion: The results of the study allow to examine.the recombinant chimeric polypeptide OspCgar+afz as a pos- sible component during creation of test-systems for serodiagnostics of LB on the territory of West, Siberia. |
| Databáze: | MEDLINE |
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