In utero exposure to ultrafine particles promotes placental stress-induced programming of renin-angiotensin system-related elements in the offspring results in altered blood pressure in adult mice.

Autor: Morales-Rubio RA; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México., Alvarado-Cruz I; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México., Manzano-León N; Departamento de Investigación Básica, Instituto Nacional de Cancerología, Ciudad de México, México., Andrade-Oliva MD; Departamento de Fisiología, Biofísica y Neurociencias, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México., Uribe-Ramirez M; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México., Quintanilla-Vega B; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México., Osornio-Vargas Á; Department of Pediatrics, University of Alberta, Edmonton, Alberta, Canada., De Vizcaya-Ruiz A; Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México, México. avizcaya@cinvestav.mx.
Jazyk: angličtina
Zdroj: Particle and fibre toxicology [Part Fibre Toxicol] 2019 Jan 28; Vol. 16 (1), pp. 7. Date of Electronic Publication: 2019 Jan 28.
DOI: 10.1186/s12989-019-0289-1
Abstrakt: Background: Exposure to particulate matter (PM) is associated with an adverse intrauterine environment, which can promote adult cardiovascular disease (CVD) risk. Ultrafine particles (UFP) (small size and large surface area/mass ratio) are systemically distributed, induce inflammation and oxidative stress, and have been associated with vascular endothelial dysfunction and arterial vasoconstriction, increasing hypertension risk. Placental stress and alterations in methylation of promoter regions of renin-angiotensin system (RAS)-related elements could be involved in UFP exposure-related programming of hypertension. We investigated whether in utero UFP exposure promotes placental stress by inflammation and oxidative stress, alterations in hydroxysteroid dehydrogenase 11b-type 2 (HSD11B2) and programming of RAS-related elements, and result in altered blood pressure in adult offspring. UFP were collected from ambient air using an aerosol concentrator and physicochemically characterized. Pregnant C57BL/6J p un /p un female mice were exposed to collected UFP (400 μg/kg accumulated dose) by intratracheal instillation and compared to control (nonexposed) and sterile H 2 O (vehicle) exposed mice. Embryo reabsorption and placental stress by measurement of the uterus, placental and fetal weights, dam serum and fetal cortisol, placental HSD11B2 DNA methylation and protein levels, were evaluated. Polycyclic aromatic hydrocarbon (PAH) biotransformation (CYP1A1 and NQO1 (NAD(P)H dehydrogenase (quinone)1)) enzymes, inflammation and oxidative stress in placentas and fetuses were measured. Postnatal day (PND) 50 in male offspring blood pressure was measured. Methylation and protein expression of (RAS)-related elements, angiotensin II receptor type 1 (AT 1 R) and angiotensin I-converting enzyme (ACE) in fetuses and lungs of PND 50 male offspring were also assessed.
Results: In utero UFP exposure induced placental stress as indicated by an increase in embryo reabsorption, decreases in the uterus, placental, and fetal weights, and HSD11B2 hypermethylation and protein downregulation. In utero UFP exposure induced increases in the PAH-biotransforming enzymes, intrauterine oxidative damage and inflammation and stimulated programming and activation of AT 1 R and ACE, which resulted in increased blood pressure in the PND 50 male offspring.
Conclusions: In utero UFP exposure promotes placental stress through inflammation and oxidative stress, and programs RAS-related elements that result in altered blood pressure in the offspring. Exposure to UFP during fetal development could influence susceptibility to CVD in adulthood.
Databáze: MEDLINE
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