The use of a microwave-assisted solvent extraction coupled with HPLC-UV/PAD to assess the quality of Marrubium vulgare L. (white horehound) herbal raw material.

Autor: Martino E; Department of Earth and Environmental Sciences, University of Pavia, Pavia, Italy., Della Volpe S; Department of Drug Sciences, University of Pavia, Pavia, Italy., Cavalloro V; Department of Earth and Environmental Sciences, University of Pavia, Pavia, Italy., Amri B; Département de Biologie, Faculté des Sciences de Tunis el Manar, Unité de recherche 'Nutrition et métabolismes azoté et protéines de stress', Tunis, Tunisia., Kaab LBB; Département de Biologie, Faculté des Sciences de Tunis el Manar, Unité de recherche 'Nutrition et métabolismes azoté et protéines de stress', Tunis, Tunisia., Marrubini G; Department of Drug Sciences, University of Pavia, Pavia, Italy., Rossi D; Department of Drug Sciences, University of Pavia, Pavia, Italy., Collina S; Department of Drug Sciences, University of Pavia, Pavia, Italy.
Jazyk: angličtina
Zdroj: Phytochemical analysis : PCA [Phytochem Anal] 2019 Jul; Vol. 30 (4), pp. 377-384. Date of Electronic Publication: 2019 Jan 27.
DOI: 10.1002/pca.2820
Abstrakt: Introduction: Marrubium vulgare is a herbal remedy presents in several European Pharmacopoeias and commonly marketed as white horehound. The chemotaxonomic marker of Marrubium genus is marrubiin and its content may change in response to biotic and abiotic stress.
Objective: Development of a microwave-assisted solvent extraction (MASE) methodology suitable for exhaustively extracting marrubiin from M. vulgare leaves, easily applicable to large sets of samples. Evaluation of the influence of copper(II) on marrubiin production.
Material and Methods: M. vulgare leaves were dried, extracted exploiting MASE and analysed via high-performance liquid chromatography ultraviolet photodiode array detection (HPLC-UV/PAD) system. A design of experiments approach was adopted to select the best extraction conditions. Extraction parameters (solvent composition, extraction time and temperature), were studied applying two full factorial experimental designs in a sequential approach. To analyse samples, a rapid HPLC-UV/PAD method was set up.
Results: The best results in terms of marrubiin extraction yield were obtained extracting samples at 120°C with 100% ethanol, for 15 min (3 × 5 min microwave cycles). The developed methodology was successfully applied to matrices grown in Greenhouse conditions and under stress induced by copper(II), selected as model agent for abiotic stress. Progressively decreasing production of marrubiin was evidenced in connection with treatment with 80, 200 and 300 mg/L copper sulphate.
Conclusion: An efficient methodology for the extraction and determination of the amount of marrubiin in large sets of samples of M. vulgare plants was developed. Results demonstrated that marrubiin is an easily detectable marker useful for evaluating M. vulgare reaction to stress.
(© 2019 John Wiley & Sons, Ltd.)
Databáze: MEDLINE
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