| Autor: |
Mulenga RM; Zambia Agriculture Research Institute, Mount Makulu Central Research Station, Chilanga, Lusaka, Zambia., Legg JP; International Institute of Tropical Agriculture, Dar es Salaam, Tanzania., Ndunguru J; Mikocheni Agriculture Research Institute, Dar es Salaam, Tanzania., Miano DW; University of Nairobi, College of Agriculture and Veterinary Sciences, Department of Plant Sciences and Crop Protection, Kangemi, Nairobi, Kenya., Mutitu EW; University of Nairobi, College of Agriculture and Veterinary Sciences, Department of Plant Sciences and Crop Protection, Kangemi, Nairobi, Kenya., Chikoti PC; Zambia Agriculture Research Institute, Mount Makulu Central Research Station., Alabi OJ; Department of Plant Pathology & Microbiology, Texas A&M AgriLife Research and Extension Center, Weslaco 78596. |
| Jazyk: |
angličtina |
| Zdroj: |
Plant disease [Plant Dis] 2016 Jul; Vol. 100 (7), pp. 1379-1387. Date of Electronic Publication: 2016 Apr 08. |
| DOI: |
10.1094/PDIS-10-15-1170-RE |
| Abstrakt: |
A survey was conducted from April to May 2014 in 214 farmers' fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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